Evaluation of sample pooling for SARS-CoV-2 RNA detection in nasopharyngeal swabs and salivas on the Roche Cobas 6800 Journal Article

   


Authors: McMillen, T.; Jani, K.; Babady, N. E.
Article Title: Evaluation of sample pooling for SARS-CoV-2 RNA detection in nasopharyngeal swabs and salivas on the Roche Cobas 6800
Abstract: The Roche Cobas SARS-CoV-2 test recently received an Emergency Use Authorization from the U.S. Food and Drug Administration UA for pooling of up to six nasopharyngeal swab samples (NPS). We evaluated the 6-pool approach on both NPS and saliva samples using 564 samples (20 positive NPS and saliva samples each and 262 negative NPS and saliva samples each). The sensitivity of the Roche SARS-CoV-2 RNA test for pooled NPS samples was 100 % (95 %CI: 83.2–100 %) and the sensitivity for pooled saliva samples was 90 % (95 % CI: 68.3–98.8 %). Given the high throughput of the Roche Cobas 6800, pooling of 6 samples has the potential to significantly increase testing capacity without significant loss in sensitivity. © 2021 Elsevier B.V.
Keywords: saliva; sars-cov-2; rt-pcr eua; sample pooling
Journal Title: Journal of Clinical Virology
Volume: 138
ISSN: 1386-6532
Publisher: Elsevier B.V.  
Date Published: 2021-05-01
Start Page: 104790
Language: English
DOI: 10.1016/j.jcv.2021.104790
PROVIDER: scopus
PMCID: PMC7944801
PUBMED: 33770658
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-85102854571&doi=10.1016%2fj.jcv.2021.104790&partnerID=40&md5=04aecc300468626c287d8e565620ce5f
Notes: Article -- Export Date: 1 April 2021 -- Source: Scopus
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MSK Authors
  1. Ngolela Esther Babady
    171 Babady
  2. Tracy Ann McMillen
    52 McMillen
  3. Krupa Vijaykumar Jani
    27 Jani
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