Interactions between Mei4, Rec114, and other proteins required for meiotic DNA double-strand break formation in Saccharomyces cerevisiae Journal Article

Authors: Maleki, S.; Neale, M. J.; Arora, C.; Henderson, K. A.; Keeney, S.
Article Title: Interactions between Mei4, Rec114, and other proteins required for meiotic DNA double-strand break formation in Saccharomyces cerevisiae
Abstract: In most sexually reproducing organisms, meiotic recombination is initiated by DNA double-strand breaks (DSBs) formed by the Spo11 protein. In budding yeast, nine other proteins are also required for DSB formation, but the roles of these proteins and the interactions among them are poorly understood. We report further studies of the behaviors of these proteins. Consistent with other studies, we find that Mei4 and Rec114 bind to chromosomes from leptonema through early pachynema. Both proteins showed only limited colocalization with the meiotic cohesin subunit Rec8, suggesting that Mei4 and Rec114 associated preferentially with chromatin loops. Rec114 localization was independent of other DSB factors, but Mei4 localization was strongly dependent on Rec114 and Mer2. Systematic deletion analysis identified protein regions important for a previously described two-hybrid interaction between Mei4 and Rec114. We also report functional characterization of a previously misannotated 5′ coding exon of REC102. Sequences encoded in this exon are essential for DSB formation and for Rec102 interaction with Rec104, Spo11, Rec114, and Mei4. Finally, we also examined genetic requirements for a set of previously described two-hybrid interactions that can be detected only when the reporter strain is induced to enter meiosis. This analysis reveals new functional dependencies for interactions among the DSB proteins. Taken together, these studies support the view that Mei4, Rec114, and Mer2 make up a functional subgroup that is distinct from other subgroups of the DSB proteins: Spo11-Ski8, Rec102-Rec104, and Mre11-Rad50-Xrs2. These studies also suggest that an essential function of Rec102 and Rec104 is to connect Mei4 and Rec114 to Spo11. © Springer-Verlag 2007.
Keywords: unclassified drug; gene sequence; exon; gene deletion; nonhuman; protein localization; protein analysis; cohesin; pachytene; synaptonemal complex; meiosis; mre11 protein; rad50 protein; protein protein interaction; protein binding; dna strand breakage; double stranded dna; saccharomyces cerevisiae; 5' untranslated region; chromatin; dna breaks, double-stranded; chromosome breakage; saccharomyces cerevisiae proteins; saccharomycetales; saccharomyces cerevisiae protein; two hybrid system; pachytene stage; spo11 protein; esterases; dna, fungal; fungal strain; protein mer2; protein mei4; protein rec102; protein rec104; protein rec114; protein rec8; protein ski8; protein xrs2
Journal Title: Chromosoma
Volume: 116
Issue: 5
ISSN: 0009-5915
Publisher: Springer  
Date Published: 2007-10-01
Start Page: 471
End Page: 486
Language: English
DOI: 10.1007/s00412-007-0111-y
PUBMED: 17558514
PROVIDER: scopus
PMCID: PMC2084462
Notes: --- - "Cited By (since 1996): 29" - "Export Date: 17 November 2011" - "CODEN: CHROA" - "Source: Scopus"
Citation Impact
MSK Authors
  1. Scott N Keeney
    119 Keeney
  2. Matthew John Neale
    5 Neale