Labeling of sarcoma associated monoclonal antibody with (111)In, (67)Ga and (125)I Journal Article
| Authors: | Lavie, E.; Boazi, M.; Weininger, J.; Bitton, M.; Yosilevski, G.; Front, D.; Hirshaut, Y.; Robinson, E.; Bartal, A. H. |
| Article Title: | Labeling of sarcoma associated monoclonal antibody with (111)In, (67)Ga and (125)I |
| Abstract: | Labeling of human sarcoma-associated murine monoclonal antibody (MAb) 23H7 with 67Ga and 111In by the bifunctional ligand method is reported. 67Ga was chelated to the MAb via desferrioxamine B and 111In via the cyclic anhydride of DTPA. Higher specific activity was obtained with 67Ga (4-5 μCi/μg) as compared with 111In (2 μCi/μg). The binding capacity of the MAb was confirmed by repeated indirect immuno-fluorescence assays performed before and after labeling. A fast blood clearance was observed: 33% recovered dose (R.D.) blood level 3 h post-injection as compared with 56% after injection of control polyclonal IgG. Preliminary results on chemically induced sarcoma bearing mice showed a relatively high tumor uptake of the labeled antibody. © 1987 Elsevier Science Publishers B.V. (Biomedical Division). |
| Keywords: | unclassified drug; human cell; animals; mice; immunofluorescence; monoclonal antibody; drug receptor binding; sarcoma; soft tissue; antibodies, monoclonal; isotope labeling; tissue distribution; iodine radioisotopes; radioisotope; radioisotopes; antibodies, neoplasm; deferoxamine mesylate; deferoxamine; sarcoma, experimental; indium; gallium radioisotopes; monoclonal antibody in 111; humans; human; priority journal; monoclonal antibody i 125; monoclonal antibody labeling; monoclonal antibody ga 67 |
| Journal Title: | Radiotherapy and Oncology |
| Volume: | 8 |
| Issue: | 2 |
| ISSN: | 0167-8140 |
| Publisher: | Elsevier Inc. |
| Date Published: | 1987-01-01 |
| Start Page: | 129 |
| End Page: | 135 |
| Language: | English |
| DOI: | 10.1016/s0167-8140(87)80166-4 |
| PUBMED: | 3562892 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 5 February 2021 -- Source: Scopus |
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