Simian virus 40 large tumor antigen requires three core replication origin domains for DNA unwinding and replication in vitro Journal Article
| Authors: | Dean, F. B.; Borowiec, J. A.; Ishimi, Y.; Deb, S.; Tegtmeyer, P.; Hurwitz, J. |
| Article Title: | Simian virus 40 large tumor antigen requires three core replication origin domains for DNA unwinding and replication in vitro |
| Abstract: | Simian virus 40 (SV40) large tumor antigen (T antigen) unwinds DNA containing the SV40 origin of replication. The origin requirement for unwinding can be satisfied by the 64-base-pair SV40 core origin that supports T-antigen-dependent DNA replication both in vivo and in vitro. The core origin contains three domains with specific DNA sequence features. These include an inverted repeat, a central T-antigen binding domain, and an adenine- and thymine-rich domain containing a DNA bending focus. The domain and spacer requirements of the core origin for DNA unwinding and replication in vitro are strikingly similar to the origin requirements for DNA replication in vivo. Thus, each of the three functional domains of the core origin contributes to the initiation of duplex DNA unwinding by T antigen. |
| Keywords: | human cell; mutation; nonhuman; dna replication; tumor antigen; genetic engineering; cell culture; escherichia coli; simian virus 40; simiae; simian virus |
| Journal Title: | Proceedings of the National Academy of Sciences of the United States of America |
| Volume: | 84 |
| Issue: | 23 |
| ISSN: | 0027-8424 |
| Publisher: | National Academy of Sciences |
| Date Published: | 1987-12-01 |
| Start Page: | 8267 |
| End Page: | 8271 |
| Language: | English |
| DOI: | 10.1073/pnas.84.23.8267 |
| PUBMED: | 2825183 |
| PROVIDER: | scopus |
| PMCID: | PMC299523 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 5 February 2021 -- Source: Scopus |
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