Monoclonal antibody to an intracellular antigen images human melanoma transplants in nu/nu mice Journal Article


Authors: Welt, S.; Mattes, M. J.; Grando, R.; Thomson, T. M.; Leonard, R. W.; Zanzonico, P. B.; Bigler, R. E.; Yeh, S.; Oettgen, H. F.; Old, L. J.
Article Title: Monoclonal antibody to an intracellular antigen images human melanoma transplants in nu/nu mice
Abstract: Mouse monoclonal antibody TA99 detects a 70-kDa pigmentation-associated glycoprotein in human melanoma cell lines. The antigen cannot be detected on the cell surface by sensitive rosetting techniques or absorption studies, nor can it be detected as a secreted product in culture fluids. Contrary to expectation, 125I-labeled TA99 specifically localized to pigmented human melanoma transplants in nu/nu mice; no localization to nonpigmented melanoma or control tumors was found. Tumor imaging was initially obscured by circulating 125I-labeled TA99 during the first 6 days after antibody injection. With clearance of 125I-labeled TA99 from the blood (half-life, 4-7 days), specific tumor images could be clearly defined by day 13. Due to the persistence of 125I-labeled TA99 at the tumor site (8.9% of the injected dose at 1 week and 4.6% at 8-10 weeks), images were obtainable for up to 10 weeks. At 8-10 weeks, the tumor/blood ratio was 104-105, and the tumor/normal tissue ratio ranged from 102 to 105. In view of these findings, antibodies detecting intracellular antigens may have a role in tumor imaging and therapy.
Keywords: human cell; nonhuman; animal cell; mouse; animals; mice; melanoma; cell line; animal experiment; animalia; monoclonal antibody; antibodies, monoclonal; antigens, neoplasm; iodine radioisotopes; mice, nude; melanoma cell; transplantation, heterologous; neoplasm transplantation; glycoprotein; cancer graft; antigen-antibody complex; humans; priority journal
Journal Title: Proceedings of the National Academy of Sciences of the United States of America
Volume: 84
Issue: 12
ISSN: 0027-8424
Publisher: National Academy of Sciences  
Date Published: 1987-06-01
Start Page: 4200
End Page: 4204
Language: English
DOI: 10.1073/pnas.84.12.4200
PUBMED: 3473501
PROVIDER: scopus
PMCID: PMC305052
DOI/URL:
Notes: Article -- Export Date: 5 February 2021 -- Source: Scopus
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MSK Authors
  1. Samuel D J Yeh
    107 Yeh
  2. Herbert F Oettgen
    130 Oettgen
  3. Lloyd J Old
    593 Old
  4. Sydney   Welt
    98 Welt