Monoclonal antibody to an intracellular antigen images human melanoma transplants in nu/nu mice Journal Article
| Authors: | Welt, S.; Mattes, M. J.; Grando, R.; Thomson, T. M.; Leonard, R. W.; Zanzonico, P. B.; Bigler, R. E.; Yeh, S.; Oettgen, H. F.; Old, L. J. |
| Article Title: | Monoclonal antibody to an intracellular antigen images human melanoma transplants in nu/nu mice |
| Abstract: | Mouse monoclonal antibody TA99 detects a 70-kDa pigmentation-associated glycoprotein in human melanoma cell lines. The antigen cannot be detected on the cell surface by sensitive rosetting techniques or absorption studies, nor can it be detected as a secreted product in culture fluids. Contrary to expectation, 125I-labeled TA99 specifically localized to pigmented human melanoma transplants in nu/nu mice; no localization to nonpigmented melanoma or control tumors was found. Tumor imaging was initially obscured by circulating 125I-labeled TA99 during the first 6 days after antibody injection. With clearance of 125I-labeled TA99 from the blood (half-life, 4-7 days), specific tumor images could be clearly defined by day 13. Due to the persistence of 125I-labeled TA99 at the tumor site (8.9% of the injected dose at 1 week and 4.6% at 8-10 weeks), images were obtainable for up to 10 weeks. At 8-10 weeks, the tumor/blood ratio was 104-105, and the tumor/normal tissue ratio ranged from 102 to 105. In view of these findings, antibodies detecting intracellular antigens may have a role in tumor imaging and therapy. |
| Keywords: | human cell; nonhuman; animal cell; mouse; animals; mice; melanoma; cell line; animal experiment; animalia; monoclonal antibody; antibodies, monoclonal; antigens, neoplasm; iodine radioisotopes; mice, nude; melanoma cell; transplantation, heterologous; neoplasm transplantation; glycoprotein; cancer graft; antigen-antibody complex; humans; priority journal |
| Journal Title: | Proceedings of the National Academy of Sciences of the United States of America |
| Volume: | 84 |
| Issue: | 12 |
| ISSN: | 0027-8424 |
| Publisher: | National Academy of Sciences |
| Date Published: | 1987-06-01 |
| Start Page: | 4200 |
| End Page: | 4204 |
| Language: | English |
| DOI: | 10.1073/pnas.84.12.4200 |
| PUBMED: | 3473501 |
| PROVIDER: | scopus |
| PMCID: | PMC305052 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 5 February 2021 -- Source: Scopus |
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