Diagnosis and monitoring in patients with hairy cell leukemia using the monoclonal antibody anti-HC2 Journal Article
| Authors: | Berman, E.; Posnett, D. N. |
| Article Title: | Diagnosis and monitoring in patients with hairy cell leukemia using the monoclonal antibody anti-HC2 |
| Abstract: | Immunofluorescent staining of peripheral blood mononuclear cells with the monoclonal antibody anti-HC2 combined with phase microscopic examination identified leukemic hairy cells in nine of 13 patients (69%) evaluated at Memorial Hospital prior to treatment with recombinant α-interferon (rIFN-αA). The remaining four patients required further studies of bone marrow or peripheral blood cytospin samples for diagnosis. In some cases a low percentage of cells staining with anti-HC2 could be significantly increased by depleting T cells from the sample using sheep red blood cell rosetting. These 13 patients represent a subgroup of patients treated on a phase II rIFN-αA study at Memorial Hospital. Ten patients (77%) achieved a partial response in a median of 128 days (range, 64-234). One patient achieved a minor response and two patients failed treatment. Nonhematologic toxicity, consisting of fever and malaise, was transient in all patients. Serial determinations of HC2-positive cells in the peripheral blood closely paralleled disease activity in the bone marrow in one patient treated for 4 years with various therapeutic modalities. The antibody anti-HC2 may play a significant role in the diagnosis and monitoring of hairy cell leukemia using peripheral blood sampling. |
| Keywords: | adult; clinical article; aged; human cell; alpha interferon; monoclonal antibody; antibodies, monoclonal; hairy cell leukemia; middle age; interferon type i; leukemia, hairy cell; human; male; female; support, u.s. gov't, p.h.s. |
| Journal Title: | Leukemia |
| Volume: | 1 |
| Issue: | 4 |
| ISSN: | 0887-6924 |
| Publisher: | Nature Publishing Group |
| Date Published: | 1987-04-01 |
| Start Page: | 305 |
| End Page: | 307 |
| Language: | English |
| PUBMED: | 3669750 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 5 February 2021 -- Source: Scopus |
