Rapid identification using a specific DNA probe of Mycobacterium avium complex from patients with acquired immunodeficiency syndrome Journal Article

   


Authors: Kiehn, T. E.; Edwards, F. F.
Article Title: Rapid identification using a specific DNA probe of Mycobacterium avium complex from patients with acquired immunodeficiency syndrome
Abstract: Specific DNA probes (Gen-Probe Corp., San Diego, Calif.) for Mycobacterium avium, Mycobacterium intracellulare, and Mycobacterium tuberculosis were compared with conventional methods for the identification of isolates of the Mycobacterium avium complex. A total of 56 isolats of M. avium complex were recovered from 34 respiratory, 13 blood, 6 stool, and 3 urine samples from 23 patients. A total of 33 isolates were tested directly from Middlebrook 7H11 agar plates, and 23 isolates were tested directly from BACTEC radiometric 12B bottles (Johnston Laboratories, Inc., Towson, Md.). Of the 56 M. avium complex isolates, 41 tested positive with the M. avium probe, 4 were positive with the M. intracellulare probe, and 7 were positive with both probes. Four direct tests from BACTEC bottles were initially negative but were subsequently M. avium probe positive when cultures from Lowenstein-Jensen agar were tested. All 56 strains were negative when tested with the M. tuberculosis probe.
Keywords: mycobacterium tuberculosis; acquired immune deficiency syndrome; mycobacterium; mycobacterium avium; priority journal; dna probe; mycobacterium avium complex; mycobacterium intracellulare
Journal Title: Journal of Clinical Microbiology
Volume: 25
Issue: 8
ISSN: 0095-1137
Publisher: American Society for Microbiology  
Date Published: 1987-08-01
Start Page: 1551
End Page: 1552
Language: English
DOI: 10.1128/jcm.25.8.1551-1552.1987
PUBMED: 3624446
PROVIDER: scopus
PMCID: PMC269268
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0023190826&doi=10.1128%2fjcm.25.8.1551-1552.1987&partnerID=40&md5=b270b64fbd9cb94f5bd8f28dfabe56e2
Notes: Article -- Export Date: 5 February 2021 -- Source: Scopus
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MSK Authors
  1. Timothy E Kiehn
    101 Kiehn
  2. Fitzroy F Edwards
    23 Edwards
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