Functional equivalents of interferon-mediated signals needed for induction of an mRNA can be generated by double-stranded RNA and growth factors Journal Article
| Authors: | Tiwari, R. K.; Kusari, J.; Sen, G. C. |
| Article Title: | Functional equivalents of interferon-mediated signals needed for induction of an mRNA can be generated by double-stranded RNA and growth factors |
| Abstract: | In our earlier studies we demonstrated that in HeLaM cells, interferon-alpha produces two functionally distinguishable signals, both of which are needed for induced transcription of mRNA 561 and other inducible mRNAs. Interferon-gamma cannot induce mRNA 561 because it produces only signal 1. Here we report that platelet-derived growth factor or epidermal growth factor could also produce signal 1. On the other hand, signal 2, which can be produced by interferon-alpha but not by interferon-gamma, could be elicited also by double-stranded RNA. Several lines of evidence suggest that the production of signal 2 by double-stranded RNA was not mediated through interferon. Interferon-induced transcription of mRNA 561 in HeLaM cells or in human fibroblast GM2767 cells was transient. However, in interferon-alpha-treated GM2767 cells, which had ceased to synthesize mRNA 561, transcription of this mRNA could be induced effectively by double-stranded RNA suggesting that this induction process could bypass the interferon-mediated down-regulation of induced transcription. Unlike HeLaM and GM2767 cells, in Daudi cells, induction of mRNA 561 by interferon-alpha was not transient. Transcription of this and two other induced mRNAs continued at a high rate even after 18 h of interferon-alpha treatment of these cells. The lack of down-regulation of interferon-induced gene expression may be responsible for interferon's acute antigrowth effects on these cells. |
| Keywords: | epidermal growth factor; platelet derived growth factor; genetics; interferon; metabolism; cell line; genetic transcription; transcription, genetic; drug effect; hela cell; hela cells; biosynthesis; gamma interferon; messenger rna; rna, messenger; platelet-derived growth factor; polyinosinic polycytidylic acid; poly i-c; double stranded rna; rna, double-stranded; growth substances; interferon type i; vesicular stomatitis virus; interferon type ii; growth promotor; humans; human; article; vesicular stomatitis-indiana virus |
| Journal Title: | EMBO Journal |
| Volume: | 6 |
| Issue: | 11 |
| ISSN: | 0261-4189 |
| Publisher: | Wiley Blackwell |
| Date Published: | 1987-11-01 |
| Start Page: | 3373 |
| End Page: | 3378 |
| Language: | English |
| DOI: | 10.1002/j.1460-2075.1987.tb02659.x |
| PUBMED: | 2828026 |
| PROVIDER: | scopus |
| PMCID: | PMC553793 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 5 February 2021 -- Source: Scopus |
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