Activated N-ras controls the transformed phenotype of HT1080 human fibrosarcoma cells Journal Article
| Authors: | Paterson, H.; Reeves, B.; Brown, R.; Hall, A.; Furth, M.; Bos, J.; Jones, P.; Marshall, C. |
| Article Title: | Activated N-ras controls the transformed phenotype of HT1080 human fibrosarcoma cells |
| Abstract: | To investigate whether the activated N-ras oncogene of HT1080 human fibrosarcoma cells contributes to the expression of the transformed phenotype, we have isolated flat revertants. In two independent revertant lines, an increase in chromosomal ploidy occurred without a concomitant increase in the number of copies of the N-ras transforming allele. Immunoprecipitation confirms that the level of the mutant N-ras p21 gene product in the revertants is correspondingly lower than in HT1080. Analysis of sporadic tumors derived from the revertant cells reveals an increased dosage of the transforming allele. The revertants also retransform after transfection of cloned activated ras oncogenes. These results imply direct participation of an N-ras oncogene in maintaining the transformed phenotype of a human tumor cell line. © 1987. |
| Keywords: | controlled study; oncoprotein; human cell; genetics; mutation; phenotype; cytology; metabolism; allele; cell line; alleles; cell transformation, neoplastic; gene expression regulation; fibrosarcoma; cell culture; cell transformation; tumor cell; oncogene protein p21(ras); genes, ras; protein p21; oncogene ras; chromosome map; chromosome mapping; oncogene n ras; oncogene proteins, viral; human; article; support, non-u.s. gov't; cell strain ht 1080 |
| Journal Title: | Cell |
| Volume: | 51 |
| Issue: | 5 |
| ISSN: | 0092-8674 |
| Publisher: | Cell Press |
| Date Published: | 1987-12-04 |
| Start Page: | 803 |
| End Page: | 812 |
| Language: | English |
| DOI: | 10.1016/0092-8674(87)90103-6 |
| PUBMED: | 3315232 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 5 February 2021 -- Source: Scopus |
