Chromosomal localization of three genes coamplified in the multidrug-resistant CH(R)C5 Chinese hamster ovary cell line Journal Article
| Authors: | Jongsma, A. P. M.; Spengler, B. A.; Van der Bliek, A. M.; Borst, P.; Biedler, J. L. |
| Article Title: | Chromosomal localization of three genes coamplified in the multidrug-resistant CH(R)C5 Chinese hamster ovary cell line |
| Abstract: | At least five gene classes are amplified in the multidrug-resistant CHO cell line CHRC5. Protein products have been identified for two classes; class 2 codes for the large membrane P-glycoprotein, whereas class 4 encodes the small cytoplasmic calcium-binding protein sorcin (VI9). By DNA analysis we have shown previously that these five genes are linked in two groups: class 1 + 2 + 3; and class 4 + 5. By use of in situ hybridization with complementary DNAs derived from the resistant cell line we demonstrate here that genes from both linkage groups are amplified and situated together in each of two different chromosomal regions of the resistant Chinese hamster cell line. The positions of the amplicons correspond to cytogenetically identified homogeneously staining regions in an altered 7q+ chromosome and in a rearranged Z-7 |t(3;4)] chromosome. The native genes were mapped both in the CH“C5 line and in a normal diploid Chinese hamster cell strain, CHNF 86. We confirm the position of the class 2 gene on lq26 and we show that class 4 and 5 genes are located in the same region of lq. We conclude that the gene classes 2, 4, and 5 are closely juxtaposed in the normal Chinese hamster genome and comprise one amplicon in resistant cells. Our results are compatible with the hypothesis that multidrug resistance is due to overexpression of P-glycoprotein genes and that the other genes amplified in the CHRC5 line are coamplified because they happen to lie close to the P-glycoprotein genes. © 1987, American Association for Cancer Research. All rights reserved. |
| Keywords: | animals; heredity; gene amplification; drug resistance; ovary; chromosome aberration; gene mapping; chromosome aberrations; nucleic acid hybridization; linkage (genetics); ovary cell; chromosome mapping; female genital system; cricetinae; hamster; female; priority journal |
| Journal Title: | Cancer Research |
| Volume: | 47 |
| Issue: | 11 |
| ISSN: | 0008-5472 |
| Publisher: | American Association for Cancer Research |
| Date Published: | 1987-06-01 |
| Start Page: | 2875 |
| End Page: | 2878 |
| Language: | English |
| PUBMED: | 3567908 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 5 February 2021 -- Source: Scopus |
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