Sorcin (V19), a soluble acidic calcium-binding protein overproduced in multidrug-resistant cells: Identification of the protein by anti-sorcin antibody Journal Article
| Authors: | Meyers, M. B.; Schneider, K. A.; Spengler, B. A.; Chang, T. D.; Biedler, J. L. |
| Article Title: | Sorcin (V19), a soluble acidic calcium-binding protein overproduced in multidrug-resistant cells: Identification of the protein by anti-sorcin antibody |
| Abstract: | Sorcin (soluble resistance-related calcium-binding protein), an acidic (pI = 5.7) protein (Mr ∼ 20 kDa) previously designated V19, was originally identified in cells selected for high levels of resistance to vincristine. Two-dimensional gel electrophoresis and/or Western blot techniques now show sorcin to be overproduced in cells selected for resistance to actinomycin D (QUA/ADj), colchicine (CHRC5), and adriamycin (BE(2)-C/ADR). Not all cell lines selected for resistance to these drugs overproduced sorcin; e.g. cells of an independently selected actinomycin d-resistant subline of QUA, QUA/ADsx, did not contain increased amounts of sorcin. Sorcin was purified by preparative gel electrophoresis from QUA/ADj cells and used to generate specific antiserum in chickens. By Western blot analyses the antiserum was shown to recognize sorcin in QUA/ADj and in vincristine-resistant mouse and Chinese hamster lung, colchicine-resistant Chinese hamster ovary, and adriamycin-resistant human neuroblastoma lines. Low level expression of the protein was detectable in control, drug-sensitive cells. Direct binding assays with 45Ca2+ showed that sorcin was a calcium-binding protein. QUA/ADj cells contained increased numbers of double minute chromosomes (DMs), cytogenetic indicators of gene amplification. As found for two other multidrug-resistant sublines, sorcin overproduction in QUA/ ADj cells may be the result of amplification of the sorcin-encoding gene. The overproduction of this protein in multidrug-resistant cells of various species implies that sorcin plays a role in expression of the resistant phenotype. © 1987. |
| Keywords: | unclassified drug; human cell; doxorubicin; animal cell; mouse; animals; mice; heredity; gene amplification; neoplasm proteins; animal experiment; calcium; vincristine; in vitro study; drug resistance; mice, inbred c57bl; ovary; drug accumulation; neuroblastoma; neuroblastoma cell; lung; neoplasms, experimental; dactinomycin; fibroblasts; drug tissue level; calcium binding protein; calcium-binding proteins; radioisotope; antibody; drug protein binding; ovary cell; pharmacokinetics; colchicine; nervous system; female genital system; cricetinae; cricetulus; drug identification; chicken; immunoelectrophoresis; respiratory system; hamster; lung alveolus cell; humans; human; female; priority journal; article; calcium 45; sorcin; sorcin antibody; drug analysis |
| Journal Title: | Biochemical Pharmacology |
| Volume: | 36 |
| Issue: | 14 |
| ISSN: | 0006-2952 |
| Publisher: | Elsevier Inc. |
| Date Published: | 1987-07-15 |
| Start Page: | 2373 |
| End Page: | 2380 |
| Language: | English |
| DOI: | 10.1016/0006-2952(87)90606-x |
| PUBMED: | 3606647 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 5 February 2021 -- Source: Scopus; Acknowledgements: The participation of M. Gay Sargent in this work is appreciated. |
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