Demonstration of phosphatidylinositol anchors on Ly-6 molecules by specific phospholipase C digestion and gel electrophoresis in octylglucoside Journal Article

  

Authors:	Hammelburger, J. W.; Palfree, R. G. E.; Sirlin, S.; Hämmerling, U.
Article Title:	Demonstration of phosphatidylinositol anchors on Ly-6 molecules by specific phospholipase C digestion and gel electrophoresis in octylglucoside
Abstract:	Release by phosphatidylinositol-specific phospholiase C is frequently provided as evidence for membrane anchorage of a protein through phosphatidylinositol. Demonstration that the enzyme removes a lipophilic moiety from the protein is stronger evidence, and is presented here for members of the Ly-6 family of lymphocyte antigens: Ly-6A, C and E. Treatment of these molecules with the enzyme greatly increased their electrophoretic mobilities on polyacrylamide gels containing nonionic detergent. Furthermore, the mobilities of the digested, but not native Ly-6 molecules, were independent of detergent. This analytical method was applied to pure antigen, radiolabelled immunoprecipitate, or immunochemically detected Ly-6 antigens on blots. © 1987 Academic Press, Inc.
Keywords:	mouse; animal; mice; mice, inbred strains; enzyme immunoassay; immunoenzyme techniques; diagnostic agent; mouse strain; antigens, ly; ly antigen; polyacrylamide gel electrophoresis; electrophoresis, polyacrylamide gel; detergent; detergents; phosphatidylinositol; phosphatidylinositols; phospholipase c; article; support, u.s. gov't, p.h.s.; glucoside; octyl beta glucoside; octyl-beta-d-glucoside; glucosides
Journal Title:	Biochemical and Biophysical Research Communications
Volume:	148
Issue:	3
ISSN:	0006-291X
Publisher:	Elsevier Science, Inc.
Date Published:	1987-11-13
Start Page:	1304
End Page:	1311
Language:	English
DOI:	10.1016/s0006-291x(87)80275-9
PUBMED:	3318830
PROVIDER:	scopus
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-0023652816&doi=10.1016%2fS0006-291X%2887%2980275-9&partnerID=40&md5=bd46005030bd26130d1bc4b077d17b4e
Notes:	Article -- Export Date: 5 February 2021 -- Source: Scopus; Acknowledgments: The authors wish to thank Drs. Martin Low, George Cross and Judith Fox for their generous donations of PI-PLC enzymes. We are indebted to Ms. Betsy Forbes for assistance in preparing the manuscript.

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