Age-determined expression of priming protease TMPRSS2 and localization of SARS-CoV-2 in lung epithelium Journal Article
| Authors: | Schuler, B. A.; Habermann, A. C.; Plosa, E. J.; Taylor, C. J.; Jetter, C.; Negretti, N. M.; Kapp, M. E.; Benjamin, J. T.; Gulleman, P.; Nichols, D. S.; Braunstein, L. Z.; Hackett, A.; Koval, M.; Guttentag, S. H.; Blackwell, T. S.; Webber, S. A.; Banovich, N. E.; Vanderbilt COVID-19 Consortium Cohort; Human Cell Atlas Biological Network; Kropski, J. A.; Sucre, J. M. S. |
| Contributor: | Pe'er, D. |
| Article Title: | Age-determined expression of priming protease TMPRSS2 and localization of SARS-CoV-2 in lung epithelium |
| Abstract: | The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) novel coronavirus 2019 (COVID-19) global pandemic has led to millions of cases and hundreds of thousands of deaths. While older adults appear at high risk for severe disease, hospitalizations and deaths due to SARS-CoV-2 among children have been relatively rare. Integrating single-cell RNA sequencing (scRNA-seq) of developing mouse lung with temporally resolved immunofluorescence in mouse and human lung tissue, we found that expression of SARS-CoV-2 Spike protein primer TMPRSS2 was highest in ciliated cells and type I alveolar epithelial cells (AT1), and TMPRSS2 expression increased with aging in mice and humans. Analysis of autopsy tissue from fatal COVID-19 cases detected SARS-CoV-2 RNA most frequently in ciliated and secretory cells in airway epithelium and AT1 cells in peripheral lung. SARS-CoV-2 RNA was highly colocalized in cells expressing TMPRSS2. Together, these data demonstrate the cellular spectrum infected by SARS-CoV-2 in lung epithelium and suggest that developmental regulation of TMPRSS2 may underlie the relative protection of infants and children from severe respiratory illness. © 2021, American Society for Clinical Investigation. |
| Journal Title: | Journal of Clinical Investigation |
| Volume: | 131 |
| Issue: | 1 |
| ISSN: | 0021-9738 |
| Publisher: | American Society for Clinical Investigation |
| Date Published: | 2021-01-04 |
| Start Page: | e140766 |
| Language: | English |
| DOI: | 10.1172/jci140766 |
| PUBMED: | 33180746 |
| PROVIDER: | scopus |
| PMCID: | PMC7773394 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 1 February 2021 -- Source: Scopus |
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