Identification of immunohistochemical reagents for in situ protein expression analysis of coronavirus-associated changes in human tissues Journal Article
| Authors: | Szabolcs, M.; Sauter, J. L.; Frosina, D.; Geronimo, J. A.; Hernandez, E.; Selbs, E.; Rapkiewicz, A. V.; Rekhtman, N.; Baine, M. K.; Jäger, E.; Travis, W. D.; Jungbluth, A. A. |
| Article Title: | Identification of immunohistochemical reagents for in situ protein expression analysis of coronavirus-associated changes in human tissues |
| Abstract: | We studied the suitability of commercially available monoclonal antibodies (mAbs) for the immunohistochemical (IHC) detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV2) in standard archival specimens. Antibodies were screened on HEK293 cells transfected with viral nucleoprotein, S1 subunit and S2 subunit of spike protein and on untransfected cells, as well as a panel of normal tissue. Lung tissue with presence of SARS-CoV2 confirmed by in situ hybridization (ISH) was also used. A total of 7 mAbs were tested: (1) mAb 001 (Sino Biological, 40143-R001), (2) mAb 007 (Sino Biological, 40150-R007), (3) mAb 019 (Sino Biological, 40143-R019), (4) mAb 1A9 (GeneTex, GTX632604), (5) mAb ABM19C9 (Abeomics, 10-10007), (6) FIPV3-70 (Santa Cruz, SC-65653), and (7) mAb 6F10 (BioVision, A2060). Only 2 mAbs, clone 001 to the nucleoprotein and clone 1A9 to the S2 subunit spike protein displayed specific immunoreactivity. Both clones showed strong staining in the acute phase of COVID-19 pneumonia, mostly in areas of acute diffuse alveolar damage, but were not completely congruent. Viral protein was also found in kidney tubules, endothelia of multiple organs and a nasal swab of a patient with persistent SARS-CoV2 infection. The other tested reagents were either poorly reactive or demonstrated nonspecific staining in tissues and lesions not infected by SARS-CoV2. Our study demonstrates that rigid specificity testing is mandatory for the evaluation of mAbs to SARS-CoV2 and that clones 001 to nucleoprotein and 1A9 to S2 subunit spike protein are useful for the in situ detection of SARS-CoV2. © 2020 Wolters Kluwer Health, Inc. All rights reserved. |
| Keywords: | immunohistochemistry; sars-cov2/covid-19; specific monoclonal antibodies |
| Journal Title: | Applied Immunohistochemistry & Molecular Morphology |
| Volume: | 29 |
| Issue: | 1 |
| ISSN: | 1541-2016 |
| Publisher: | Lippincott Williams & Wilkins |
| Date Published: | 2021-01-01 |
| Start Page: | 5 |
| End Page: | 12 |
| Language: | English |
| DOI: | 10.1097/pai.0000000000000878 |
| PUBMED: | 33086222 |
| PROVIDER: | scopus |
| PMCID: | PMC7713639 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 4 January 2021 -- Source: Scopus |
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