High-resolution mouse subventricular zone stem-cell niche transcriptome reveals features of lineage, anatomy, and aging Journal Article


Authors: Xie, X.; Laks, D. R.; Sun, D.; Poran, A.; Laughney, A. M.; Wang, Z.; Sam, J.; Belenguer, G.; Fariñas, I.; Elemento, O.; Zhou, X.; Parada, L. F.
Article Title: High-resolution mouse subventricular zone stem-cell niche transcriptome reveals features of lineage, anatomy, and aging
Abstract: Adult neural stem cells (NSC) serve as a reservoir for brain plasticity and origin for certain gliomas. Lineage tracing and genomic approaches have portrayed complex underlying heterogeneity within the major anatomical location for NSC, the subventricular zone (SVZ). To gain a comprehensive profile of NSC heterogeneity, we utilized a well-validated stem/progenitor-specific reporter transgene in concert with single-cell RNA sequencing to achieve unbiased analysis of SVZ cells from infancy to advanced age. The magnitude and high specificity of the resulting transcriptional datasets allow precise identification of the varied cell types embedded in the SVZ including specialized parenchymal cells (neurons, glia, microglia) and noncentral nervous system cells (endothelial, immune). Initial mining of the data delineates four quiescent NSC and three progenitor-cell subpopulations formed in a linear progression. Further evidence indicates that distinct stem and progenitor populations reside in different regions of the SVZ. As stem/progenitor populations progress from neonatal to advanced age, they acquire a deficiency in transition from quiescence to proliferation. Further data mining identifies stagespecific biological processes, transcription factor networks, and cellsurface markers for investigation of cellular identities, lineage relationships, and key regulatory pathways in adult NSC maintenance and neurogenesis. © 2020 National Academy of Sciences. All rights reserved.
Keywords: neural stem cell; subventricular zone; aging; transcriptome; single-cell rna sequencing
Journal Title: Proceedings of the National Academy of Sciences of the United States of America
Volume: 117
Issue: 49
ISSN: 0027-8424
Publisher: National Academy of Sciences  
Date Published: 2020-12-08
Start Page: 31448
End Page: 31458
Language: English
DOI: 10.1073/pnas.2014389117
PUBMED: 33229571
PROVIDER: scopus
PMCID: PMC7733854
DOI/URL:
Notes: Article -- Export Date: 4 January 2021 -- Source: Scopus
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MSK Authors
  1. Luis F Parada
    28 Parada
  2. Zilai Wang
    9 Wang
  3. Daochun Sun
    15 Sun
  4. Xuanhua Xie
    12 Xie
  5. Dan R Laks
    7 Laks