Demonstration of altered cellular DNA content distribution in long-term colon epithelial cell lines with colon cancer genotypes Journal Article

  

Authors:	Shannon Danes, B.; Deangelis, P.; Traganos, F.; Melamed, M. R.; Alm, T.
Article Title:	Demonstration of altered cellular DNA content distribution in long-term colon epithelial cell lines with colon cancer genotypes
Abstract:	During prolonged culture (7-40 passages assayed) DNA distribution was constant in IVT-negative lines with FPC genotype and in IVT-negative lines without any known colon cancer genotype, whereas the DNA histograms of the IVT-positive lines with HNPCC genotype showed a marked change. The DNA index (DI) of > 1 increased sixfold in the HNPCC-A (affected) line and threefold in the HNPCC-AR (at-risk) line due to an equal increase in DI of 2 and DI of > 2. It is proposed that there is a possible association between the HNPCC genotype, the presence of IVT, and the marked increase in both DI of 2 and DI of > 2 in DNA histograms, since neither the lines with normal nor those with FPC genotypes showed these changes. The biological significance of IVT might be due to its influence on the constancy of the distribution of cellular DNA content, leading to aneuploidy, which has been known to precede in vitro tumorigenic phenotypes. © 1988 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.
Keywords:	adolescent; adult; human cell; flow cytometry; colonic neoplasms; genotype; dna; cell culture; colon cancer; dna, neoplasm; cell line, transformed; epithelium; colon; tetraploidy; polyploidy; tissue culture; cell dna; intestinal mucosa; human; male; priority journal; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; colonic epithelial lines; dna distribution; heritable colon cancer
Journal Title:	Scandinavian Journal of Gastroenterology
Volume:	23
Issue:	7
ISSN:	0036-5521
Publisher:	Informa Healthcare
Date Published:	1988-01-01
Start Page:	840
End Page:	846
Language:	English
DOI:	10.3109/00365528809090770
PUBMED:	3227300
PROVIDER:	scopus
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-0023767146&doi=10.3109%2f00365528809090770&partnerID=40&md5=4fe03b61531a7ee1223950833fcdf556
Notes:	Article -- Export Date: 6 August 2020 -- Source: Scopus

https://synapse.mskcc.org/synapse/works/has_related_data_chk/171026