Psidin is required in Drosophila blood cells for both phagocytic degradation and immune activation of the fat body Journal Article
| Authors: | Brennan, C. A.; Delaney, J. R.; Schneider, D. S.; Anderson, K. V. |
| Article Title: | Psidin is required in Drosophila blood cells for both phagocytic degradation and immune activation of the fat body |
| Abstract: | Phagocytic blood cells are critical to innate immune defense: They internalize and destroy microbial invaders and produce signals that trigger other immune responses [1, 2]. Despite this central role, the in vivo contributions of phagocytosis to systemic immune activation are not well understood. Drosophila has proven a fruitful model for the investigation of evolutionarily conserved innate immune mechanisms, including NF-κB-dependent transcriptional induction, RNAi in antiviral responses, and phagocytosis [3-5]. The phagocytes of Drosophila encounter bacterial invaders early in infection and contribute to survival of infection [6-9]. Phagocytosis in flies and mammals is highly homologous: Both rely on scavenger receptors, opsonins, and actin rearrangements for engulfment; have phagosomal cysteine proteases active at low pH; and can be subverted by similar intracellular pathogens [9-13]. Although the role of Drosophila phagocytes in the activation of other immune tissues has not been clear, we show that induction of the antibacterial-peptide gene Defensin in the fat body during infection requires blood-cell contributions. We identify a gene, psidin, that encodes a lysosomal protein required in the blood cells for both degradation of engulfed bacteria and activation of fat-body Defensin. These data establish a role for the phagocytic blood cells of Drosophila in detection of infection and activation of the humoral immune response. © 2007 Elsevier Ltd. All rights reserved. |
| Keywords: | signal transduction; unclassified drug; genetics; mutation; blood proteins; animal; metabolism; mammalia; animals; drosophila; bacteria (microorganisms); physiology; gene expression regulation; antigen presentation; immunology; cellbio; blood cell; bacteremia; phagocytosis; drosophila proteins; bacterium; phagocyte; lysosome; drosophila protein; larva; plasma protein; cellimmuno; lysosomes; bacteria; fat body; hemocytes; defensin; psidin protein, drosophila; spatzle protein, drosophila; defensins; phagocytes |
| Journal Title: | Current Biology |
| Volume: | 17 |
| Issue: | 1 |
| ISSN: | 0960-9822 |
| Publisher: | Cell Press |
| Date Published: | 2007-01-09 |
| Start Page: | 67 |
| End Page: | 72 |
| Language: | English |
| DOI: | 10.1016/j.cub.2006.11.026 |
| PUBMED: | 17208189 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 34" - "Export Date: 17 November 2011" - "CODEN: CUBLE" - "Source: Scopus" |
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