Simultaneous production of tumor necrosis factor-α and lymphotoxin by normal T cells after induction with IL-2 and anti-T3 Journal Article
| Authors: | Steffen, M.; Ottmann, O. G.; Moore, M. A. S. |
| Article Title: | Simultaneous production of tumor necrosis factor-α and lymphotoxin by normal T cells after induction with IL-2 and anti-T3 |
| Abstract: | Normal human T cells purified from the peripheral blood and cultured for 6 days in the presence of OKT3 and IL-2 were shown to produce simultaneously TNF and lymphotoxin (LT) mRNA. Both biologically active TNF and LT were detected in supernatants of the cultured T cells using WEHI 164 as target cells. The cytotoxic activities of both cytokines could be inhibited with specific antibodies against TNF or LT. The T cell cultures were free of macrophages, NK cells, or B lymphocytes as assessed by flow cytometric analysis. |
| Keywords: | adult; human cell; flow cytometry; t lymphocyte; t-lymphocytes; cells, cultured; interleukin 2; biological model; cell line; monoclonal antibody; cytokine; lymphocyte activation; antibodies, monoclonal; cell culture; cell separation; cytochemistry; interleukin-2; tumor necrosis factor; antigens, differentiation, t-lymphocyte; lymphotoxin; human; male; female; priority journal; support, non-u.s. gov't; support, u.s. gov't, p.h.s. |
| Journal Title: | Journal of Immunology |
| Volume: | 140 |
| Issue: | 8 |
| ISSN: | 0022-1767 |
| Publisher: | The American Association of Immunologists, Inc |
| Date Published: | 1988-04-15 |
| Start Page: | 2621 |
| End Page: | 2624 |
| Language: | English |
| PUBMED: | 3258616 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 6 August 2020 -- Source: Scopus |
