Synergistic inhibition of human marrow granulocyte-macrophage progenitor cells by prostaglandin E and recombinant interferon-α, -β, and -γ and an effect mediated by tumor necrosis factor Journal Article
| Authors: | Pelus, L. M.; Ottmann, O. G.; Nocka, K. H. |
| Article Title: | Synergistic inhibition of human marrow granulocyte-macrophage progenitor cells by prostaglandin E and recombinant interferon-α, -β, and -γ and an effect mediated by tumor necrosis factor |
| Abstract: | The effects of prostaglandin E (PGE) and recombinant human interferon-α, -β, and -γ alone and in combination were tested for their effects on the proliferation of human bone marrow granulocyte-macrophage colony-forming units (GM-CFU). When tested alone, both classes of cytokines inhibited GM-CFU proliferation. In combination, PGE and all three types of recombinant interferons synergized in their ability to inhibit GM-CFU proliferation. Progressive enrichment for marrow GM-CFU indicated that the synergistic effects of PGE and interferon were dependent upon the presence of marrow-adherent cells. Studies using conditioned media from marrow-adherent cells prepared in the presence of interferon-α, -β, and -γ indicated that adherent cells produced a soluble factor in the presence of interferons that subsequently synergized with PGE in inhibiting GM-CFU proliferation. Neutralization of this conditioned media with a monoclonal antibody to tumor necrosis factor abrogated the synergistic inhibition of GM-CFU observed in the presence of PGE. The addition of recombinant tumor necrosis factor and PGE to accessory cell-depleted bone marrow resulted in synergistic inhibition of GM-CFU proliferation. |
| Keywords: | adult; alpha interferon; bone marrow cells; bone marrow; cell growth; drug synergism; antibodies, monoclonal; gamma interferon; cell culture; recombinant proteins; hematopoietic stem cells; bone marrow cell; macrophages; prostaglandin e; prostaglandins e; cell adhesion; beta interferon; antigen-presenting cells; helper cell; colony forming unit gm; tumor necrosis factor; dinoprostone; interferon type i; interferon type ii; granulocytes; human; priority journal; support, non-u.s. gov't; support, u.s. gov't, p.h.s. |
| Journal Title: | Journal of Immunology |
| Volume: | 140 |
| Issue: | 2 |
| ISSN: | 0022-1767 |
| Publisher: | The American Association of Immunologists, Inc |
| Date Published: | 1988-01-15 |
| Start Page: | 479 |
| End Page: | 484 |
| Language: | English |
| PUBMED: | 3121745 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 6 August 2020 -- Source: Scopus |
