Identification of N-glycolylneuraminic acid-containing gangliosides of cat and sheep erythrocytes: (252)Cf fission fragment ionization mass spectrometry in the analysis of glycosphingolipids Journal Article
| Authors: | Furukawa, K.; Chait, B. T.; Lloyd, K. O. |
| Article Title: | Identification of N-glycolylneuraminic acid-containing gangliosides of cat and sheep erythrocytes: (252)Cf fission fragment ionization mass spectrometry in the analysis of glycosphingolipids |
| Abstract: | A number of gangliosides were isolated from cat and sheep erythrocytes for use in analyzing the specificity of a panel of human anti-heterophile monoclonal antibodies. The structures of these compounds were determined by a combination of different procedures, including sugar analysis, glycosidase treatment, periodate oxidation. TLC immunostaining, methylation analysis, and mass spectrometry. These methods identified the cat erythrocytes gangliosides (C1 and C2) as N-glycolylneuraminic acid (NeuGc)-containing hematosides; C1 was shown to be NeuGcα2 → 8 NeuGcα2 → 3Galβ1 → 4Glc-Cer ((neuGc)2G(D3)) and C2 to be NeuAcα2 → 8NeuGcα2 → 3Galβ1 → 4Glc-Cer ((NeuAc-NeuGc-)G(D3)). The two sheep gangliosides (S1 and S2) were found to be novel glycolipids based on the paragloboside sequence; S1 was identified as NeuGcα2 → 8NeuGcα2 → 3Galβ1 → 4GlcNAcβ1 → 3Galβ1 → 4Glc-Cer ((NeuGc)2-disialylparagloboside) and S2 as NeuAcα2 → 8NeuGcα2 → 3Galβ1 → 4GlcNacβ1 → 3Galβ1 → 4Glc-Cer ((NeuAc-NeuGc-)-disialylparagloboside). Structural analysis of these compounds was aided by the use of 252Cf fission fragment ionization time-of-flight mass spectrometry. This method provided easily interpretable spectra on methylated derivatives which were particularly useful in determining the sialic acid composition of the gangliosides and the sequence of their disialosyl side chains. |
| Keywords: | nonhuman; mass spectrometry; animal cell; animal; molecular sequence data; cat; cats; erythrocyte; neuraminidase; carbohydrate sequence; carbohydrates; glycosphingolipid; lipid analysis; carbohydrate conformation; gangliosides; ganglioside; glycosphingolipids; sheep; priority journal; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; spectrum analysis, mass; neuraminic acids; n glycoloylneuraminic acid; californium |
| Journal Title: | Journal of Biological Chemistry |
| Volume: | 263 |
| Issue: | 29 |
| ISSN: | 0021-9258 |
| Publisher: | American Society for Biochemistry and Molecular Biology |
| Date Published: | 1988-10-15 |
| Start Page: | 14939 |
| End Page: | 14947 |
| Language: | English |
| PUBMED: | 3170570 |
| PROVIDER: | scopus |
| DOI: | 10.1016/S0021-9258(18)68129-3 |
| DOI/URL: | |
| Notes: | Article -- Source: Scopus |
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