Role of insulin in growth hormone-stimulated 3T3 cell adipogenesis Journal Article


Authors: Guller, S.; Corin, R. E.; Mynarcik, D. C.; London, B. M.; Sonenberg, M.
Article Title: Role of insulin in growth hormone-stimulated 3T3 cell adipogenesis
Abstract: The role of insulin during GH-stimulated adipogenesis of 3T3-F442A fibroblasts was investigated. Adipogenesis in defined medium (DM), as quantified by the level of glycerol-3-phosphate dehydrogenase activity, revealed that there existed a strict requirement for both insulin and GH during adipogenesis. The concentration of insulin required to elicit halfmaximal adipogenesis was approximately 20 nM. Insulin-like growth factor I was less effective than insulin in promoting adipogenesis, indicating that insulin action during differentiation was most likely mediated through the insulin receptor. Cellular viability was not compromised by the absence of insulin, as judged by colony-forming efficiency or trypan blue exclusion. Deletion of insulin from DM supplemented with 1 nM recombinant human GH reduced glycerol-3-phosphate dehydrogenase activity to uninduced levels. Removal of other individual DM constituents did not have this effect. The growth factors fibroblast growth factor, platelet-derived growth factor, and bombesin did not substitute for insulin during GH-stimulated adipogenesis. The characteristic increase in cell number observed during serum-based differentiation, reflecting clonal expansion of young adipocytes, did not occur in DM supplemented with insulin and GH, suggesting that serum factors other than GH, insulin, and insulin-like growth factor I were necessary for this event. These results suggest that insulin functions in concert with GH as a coinducer of the differentiating signals. © 1988 by The Endocrine Society.
Keywords: nonhuman; methodology; animal cell; animal; mice; cells, cultured; animal experiment; cell differentiation; growth hormone; kinetics; mammal; insulin; somatomedin c; cell strain 3t3; adipose tissue; adipocyte; adipogenesis; support, u.s. gov't, p.h.s.; glycerol 3 phosphate dehydrogenase; glycerolphosphate dehydrogenase
Journal Title: Endocrinology
Volume: 122
Issue: 5
ISSN: 0013-7227
Publisher: Endocrine Society  
Date Published: 1988-05-01
Start Page: 2084
End Page: 2089
Language: English
DOI: 10.1210/endo-122-5-2084
PUBMED: 3282874
PROVIDER: scopus
DOI/URL:
Notes: Article -- Export Date: 6 August 2020 -- Source: Scopus
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