The human Lyt-3 molecule requires CD8 for cell surface expression Journal Article
| Authors: | DiSanto, J. P.; Knowles, R. W.; Flomenberg, N. |
| Article Title: | The human Lyt-3 molecule requires CD8 for cell surface expression |
| Abstract: | We have previously identified a monoclonal antibody, T8/2T8-5H7, which clustered serologically with CD8 monoclonal antibodies, but lacked reactivity with L cell transfectants expressing the human CD8 molecule (Lyt-2 homologue). Based on these observations, we postulated that T8/2T8-5H7 might recognize the human Lyt-3 gene product. To test this hypothesis, we have isolated a full-length cDNA encoding the human Lyt-3 molecule and have characterized its product in additional transfection experiments. The results of these studies indicate that the human Lyt-3 cDNA encodes a product recognized by the antibody T8/2T8-5H7. Interestingly, the human Lyt-3 molecule cannot be expressed alone, but requires the human Lyt-2 homologue for efficient cell surface expression. A heterodimer composed of the human Lyt-2 and Lyt-3 molecules may have importance in T cell-target cell interactions. |
| Keywords: | genetics; molecular genetics; cd8 antigen; mouse; animal; animals; mice; cell line; monoclonal antibody; gene expression regulation; fluorescent antibody technique; membrane antigen; immunology; antibodies, monoclonal; dna; molecular sequence data; nucleotide sequence; cell membrane; base sequence; antigens, ly; ly antigen; antigens, surface; antigens, cd8; t lymphocyte antigen; antigens, differentiation, t-lymphocyte; humans; human; article; l cells (cell line); l cell |
| Journal Title: | EMBO Journal |
| Volume: | 7 |
| Issue: | 11 |
| ISSN: | 0261-4189 |
| Publisher: | Wiley Blackwell |
| Date Published: | 1988-11-01 |
| Start Page: | 3465 |
| End Page: | 3470 |
| Language: | English |
| DOI: | 10.1002/j.1460-2075.1988.tb03221.x |
| PUBMED: | 3145196 |
| PROVIDER: | scopus |
| PMCID: | PMC454846 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 6 August 2020 -- Source: Scopus |
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