Regulation of murine α-, β major-, and β minor-globin gene expression Journal Article

  


Authors: Weich, N.; Marks, P. A.; Rifkind, R. A.
Article Title: Regulation of murine α-, β major-, and β minor-globin gene expression
Abstract: Transcriptional properties of the mouse globin genes and their promoters were examined in COS cells using transient expression assays. Transfected intact mouse α1-globin genes generate transcripts, whereas βmaj and βmin globin transcripts are detected only if the genes are associated with a strong exogenous (SV40) enhancer sequence. Under these conditions the ratio of accumulated βmaj and βmin mRNA sequences approximates the 4:1 ratio observed in reticulocytes and murine erythroleukemia cells (MELC) induced to differentiate by hexamethylene bisacetamide (HMBA). As determined using hybrid genes consisting of globin gene 5′ regions fused to the chloramphenicol acetyltransferase (CAT) structural gene, this 4:1 ratio appears dependent upon the relative activities of the two 5′ promoter regions. © 1988.
Keywords: animal cell; mouse; animal; mice; gene expression; transcription, genetic; transfection; gene expression regulation; dna; genetic engineering; messenger rna; rna, messenger; globin; simian virus 40; plasmids; nucleic acid hybridization; acetyltransferases; globins; leukemia, erythroblastic, acute; enhancer elements (genetics); promoter regions (genetics); mice, inbred dba; erythroleukemia; hexamethylenebisacetamide; dna, recombinant; reticulocyte; acetamides; reticulocytes; chloramphenicol acetyltransferase; chloramphenicol o-acetyltransferase; support, non-u.s. gov't; support, u.s. gov't, p.h.s.
Journal Title: Biochemical and Biophysical Research Communications
Volume: 150
Issue: 1
ISSN: 0006-291X
Publisher: Elsevier Science, Inc.  
Date Published: 1988-01-15
Start Page: 204
End Page: 211
Language: English
DOI: 10.1016/0006-291x(88)90506-2
PUBMED: 2827666
PROVIDER: scopus
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0023838305&doi=10.1016%2f0006-291X%2888%2990506-2&partnerID=40&md5=25ddd18c4b319b08d3e8ec395ea205c4
Notes: Article -- Export Date: 6 August 2020 -- Source: Scopus
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MSK Authors
  1. Paul Marks
    186 Marks
  2. Richard Rifkind
    118 Rifkind
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