EGFR blockade reverts resistance to KRAS(G12C) inhibition in colorectal cancer Journal Article


Authors: Amodio, V.; Yaeger, R.; Arcella, P.; Cancelliere, C.; Lamba, S.; Lorenzato, A.; Arena, S.; Montone, M.; Mussolin, B.; Bian, Y.; Whaley, A.; Pinnelli, M.; Murciano-Goroff, Y. R.; Vakiani, E.; Valeri, N.; Liao, W. L.; Bhalkikar, A.; Thyparambil, S.; Zhao, H. Y.; de Stanchina, E.; Marsoni, S.; Siena, S.; Bertotti, A.; Trusolino, L.; Li, B. T.; Rosen, N.; Di Nicolantonio, F.; Bardelli, A.; Misale, S.
Article Title: EGFR blockade reverts resistance to KRAS(G12C) inhibition in colorectal cancer
Abstract: Most patients with KRAS(G12C)-mutant non-small cell lung cancer (NSCLC) experience clinical benefit from selective KRAS(G12C) inhibition, whereas patients with colorectal cancer bearing the same mutation rarely respond. To investigate the cause of the limited efficacy of KRAS(G12C) inhibitors in colorectal cancer, we examined the effects of AMG510 in KRAS(G12C) colorectal cancer cell lines. Unlike NSCLC cell lines, KRAS(G12C) colorectal cancer models have high basal receptor tyrosine kinase (RTK) activation and are responsive to growth factor stimulation. In colorectal cancer lines, KRAS(G12C) inhibition induces higher phospho-ERK rebound than in NSCLC cells. Although upstream activation of several RTKs interferes with KRAS(G12C) blockade, we identify EGFR signaling as the dominant mechanism of colorectal cancer resistance to KRAS(G12C) inhibitors. The combinatorial targeting of EGFR and KRAS(G12C) is highly effective in colorectal cancer cells and patient-derived organoids and xenografts, suggesting a novel therapeutic strategy to treat patients with KRAS(G12C) colorectal cancer. SIGNIFICANCE: The efficacy of KRAS(G12C)inhibitors in NSCLC and colorectal cancer is lineage-specific. RTK dependency and signaling rebound kinetics are responsible for sensitivity or resistance to KRAS(G12C) inhibition in colorectal cancer. EGFR and KRAS(G12C) should be concomitantly inhibited to overcome resistance to KRAS(G12C) blockade in colorectal tumors.
Keywords: cetuximab; ras; trial; acquired-resistance; landscape
Journal Title: Cancer Discovery
Volume: 10
Issue: 8
ISSN: 2159-8274
Publisher: American Association for Cancer Research  
Date Published: 2020-08-01
Start Page: 1129
End Page: 1139
Language: English
ACCESSION: WOS:000558688700022
DOI: 10.1158/2159-8290.Cd-20-0187
PROVIDER: wos
PMCID: PMC7416460
PUBMED: 32430388
Notes: Article -- Source: Wos
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  1. Neal Rosen
    426 Rosen
  2. Rona Denit Yaeger
    324 Yaeger
  3. Efsevia Vakiani
    266 Vakiani
  4. Bob Tingkan Li
    279 Li
  5. HuiYong   Zhao
    26 Zhao
  6. Sandra Misale
    17 Misale
  7. Adele Simone Whaley
    2 Whaley
  8. Yu Bian
    2 Bian