Functional and genomic variation between human-derived isolates of lachnospiraceae reveals inter- and intra-species diversity Journal Article
| Authors: | Sorbara, M. T.; Littmann, E. R.; Fontana, E.; Moody, T. U.; Kohout, C. E.; Gjonbalaj, M.; Eaton, V.; Seok, R.; Leiner, I. M.; Pamer, E. G. |
| Article Title: | Functional and genomic variation between human-derived isolates of lachnospiraceae reveals inter- and intra-species diversity |
| Abstract: | Members of the Lachnospiraceae family are abundant members of the microbiota and have health-promoting functions. Sorbara et al. describe genomic and functional diversity within a large collection of cultured Lachnospiraceae isolates. Inter- and intra-species diversity has important implications for the development of therapeutic consortia. © 2020 Elsevier Inc. Bacteria belonging to the Lachnospiraceae family are abundant, obligate anaerobic members of the microbiota in healthy humans. Lachnospiraceae impact their hosts by producing short-chain fatty acids, converting primary to secondary bile acids, and facilitating colonization resistance against intestinal pathogens. To increase our understanding of genomic and functional diversity between members of this family, we cultured 273 Lachnospiraceae isolates representing 11 genera and 27 species from human donors and performed whole-genome sequencing assembly and annotation. This analysis revealed substantial inter- and intra-species diversity in pathways that likely influence an isolate's ability to impact host health. These differences are likely to impact colonization resistance through lantibiotic expression or intestinal acidification, influence host mucosal immune cells and enterocytes via butyrate production, or contribute to synergism within a consortium by heterogenous polysaccharide metabolism. Identification of these specific functions could facilitate development of probiotic bacterial consortia that drive and/or restore in vivo microbiome functions. © 2020 Elsevier Inc. |
| Keywords: | microbiota; microbiome; commensal bacteria; genomic diversity; blautia; butyrate; lachnospiraceae; clostridia; live biotherapeutics; symbiotic bacteria |
| Journal Title: | Cell Host & Microbe |
| Volume: | 28 |
| Issue: | 1 |
| ISSN: | 1931-3128 |
| Publisher: | Cell Press |
| Date Published: | 2020-07-08 |
| Start Page: | 134 |
| End Page: | 146.e4 |
| Language: | English |
| DOI: | 10.1016/j.chom.2020.05.005 |
| PUBMED: | 32492369 |
| PROVIDER: | scopus |
| PMCID: | PMC7351604 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 1 September 2020 -- Source: Scopus |
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