Chemical probes and methods for single-cell detection and quantification of epichaperomes in hematologic malignancies Journal Article

   


Authors: Merugu, S.; Sharma, S.; Kaner, J.; Digwal, C.; Sugita, M.; Joshi, S.; Taldone, T.; Guzman, M. L.; Chiosis, G.
Article Title: Chemical probes and methods for single-cell detection and quantification of epichaperomes in hematologic malignancies
Abstract: Detection of protein connectivity dysfunctions in biological samples, i.e., informing on how protein-protein interactions change from a normal to a disease state, is important for both biomedical research and clinical development. The epichaperome is an executor of protein connectivity dysfunction in disease, and thus a surrogate for its detection. This chapter will detail on published methods for epichaperome detection and quantification that combine the advantages of multiparameter flow cytometry with those of the PU-FITC fluorescently labeled epichaperome detection probe. It will offer a comprehensive method description that includes the synthesis and characterization of an epichaperome detection probe and of the negative control probe, the preparation of the biospecimen for epichaperome analysis, the execution of the epichaperome detection and quantification assay and lastly, the data acquisition and analysis. The method provides, at single-cell level, the functional signature of cells, differentiating itself from other single-cell methods that provide a catalog of molecules. © 2020 Elsevier Inc.
Keywords: hematologic malignancies; multiparameter flow cytometry; epichaperome chemical probe; protein connectivity dysfunction; single-cell proteome dysfunction detection
Journal Title: Methods in Enzymology
Volume: 639
ISSN: 0076-6879
Publisher: Academic Press  
Date Published: 2020-01-01
Start Page: 289
End Page: 311
Language: English
DOI: 10.1016/bs.mie.2020.04.057
PUBMED: 32475406
PROVIDER: scopus
PMCID: PMC7397528
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-85084478230&doi=10.1016%2fbs.mie.2020.04.057&partnerID=40&md5=7384b13248a0489848047cf2306fe9e1
Notes: Book Chapter 13 in Chenoweth DM, ed. "Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems: Diverse Methods for Optical Imaging and Conjugation" (ISBN: 978-0-12-821151-9) -- Export Date: 3 August 2020 -- Source: Scopus
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MSK Authors
  1. Gabriela Chiosis
    279 Chiosis
  2. Tony Taldone
    93 Taldone
  3. Sahil Sharma
    31 Sharma
  4. Suhasini Joshi
    20 Joshi
  5. Chander Singh Digwal
    17 Digwal
  6. Swathi Merugu
    6 Merugu
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