Isolation of a novel intertypic recombinant human mastadenovirus B2 from two unrelated bone marrow transplant recipients Journal Article
| Authors: | Kajon, A. E.; Lamson, D. M.; Spiridakis, E.; Cardenas, A. M.; Babady, N. E.; Fisher, B. T.; St George, K. |
| Article Title: | Isolation of a novel intertypic recombinant human mastadenovirus B2 from two unrelated bone marrow transplant recipients |
| Abstract: | Human adenoviruses (HAdV) are well-known opportunistic pathogens of immunocompromised adult and pediatric patients but specific associations between HAdV species or individual HAdV types and disease are poorly understood. In this study we report the isolation of a novel HAdV-B2 genotype from two unrelated immunocompromised patients, both recipients of a hematopoietic cell transplant. In both patients, the course of HAdV infection is consistent with a scenario of reactivation of a latent virus rather than a primary opportunistic infection. Archived HAdV PCR-positive plasma, urine, and stool specimens were processed for virus isolation and detailed molecular characterization. Virus isolates were recovered from patient 1 from PCR-positive urine specimens obtained at days 103 and 116 after transplant in association with gross hematuria, and from a stool specimen obtained 138 days after transplant in association with diarrhea. An isolate was recovered from patient 2 from a PCR-positive urine specimen. Hexon and fiber gene amplification and sequencing were carried out for initial molecular typing, identifying the isolates as an intertypic recombinant with a HAdV-11-like hexon gene and a HAdV-77-like fiber gene. Comprehensive restriction fragment length polymorphism (RFLP) analysis was performed on viral DNA purified from urine and stool isolates, and next generation whole genome sequencing was carried out on purified viral genomic DNA. The genomes of the two isolated strains are 99.5% identical and represent the same RFLP genomic variant. The identified virus is a novel HAdV-B2 genotype designated HAdV-78 exhibiting a HAdV-11-like penton base, a HAdV-11-like hexon and a HAdV-77-like fiber (P11H11F77). © 2020 The Authors |
| Keywords: | adolescent; adult; clinical article; middle aged; gene sequence; fludarabine; prednisone; fatigue; case report; diarrhea; rituximab; gene; gene amplification; mucosa inflammation; vomiting; bendamustine; cyclophosphamide; hematopoietic stem cell transplantation; hematuria; thiotepa; acute lymphoblastic leukemia; fever; pneumonia; whole body radiation; molecular typing; graft versus host reaction; urinary frequency; methylprednisolone; cidofovir; real time polymerase chain reaction; bacteremia; urinary urgency; drug dose increase; tacrolimus; bone marrow transplantation; chronic lymphatic leukemia; gastrointestinal endoscopy; hemorrhagic cystitis; fluconazole; cyclosporine; graft recipient; pentostatin; virus detection; dysuria; immunocompromised patient; virus isolation; valganciclovir; adenovirus; molecular diagnosis; viremia; restriction fragment length polymorphism; esophagus candidiasis; rhinorrhea; body weight loss; staphylococcus epidermidis; sanger sequencing; whole-genome sequencing; human; male; priority journal; article; whole genome sequencing; human adenovirus c; human adenovirus infection; lymphocytic lymphoma; urine sampling; genotype hadv-78; species hadv-b; fiber gene; hexon gene; paired end sequencing; recombinant human mastadenovirus b2 |
| Journal Title: | New Microbes and New Infections |
| Volume: | 35 |
| ISSN: | 2052-2975 |
| Publisher: | Elsevier Ltd |
| Date Published: | 2020-05-01 |
| Start Page: | 100677 |
| Language: | English |
| DOI: | 10.1016/j.nmni.2020.100677 |
| PROVIDER: | scopus |
| PMCID: | PMC7210458 |
| PUBMED: | 32405419 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 1 June 2020 -- Source: Scopus |
