Increased expression of histone deacetylaces (HDACs) and inhibition of prostate cancer growth and invasion by HDAC inhibitor SAHA Journal Article
| Authors: | Wang, L.; Zou, X.; Berger, A. D.; Twiss, C.; Peng, Y.; Li, Y.; Chiu, J.; Guo, H.; Satagopan, J.; Wilton, A.; Gerald, W.; Basch, R.; Wang, Z.; Osman, I.; Lee, P. |
| Article Title: | Increased expression of histone deacetylaces (HDACs) and inhibition of prostate cancer growth and invasion by HDAC inhibitor SAHA |
| Abstract: | Histone deacetetylases (HDACs) are a group of corepressors of transcriptional activators and their levels of expression are potentially dysregulated in prostate cancer. Certain inhibitors of histone deacetylases show anti tumor activity in prostate cancer cell lines. Here, we systemically studied the expression of HDACs in human prostate cancer and the suppression of prostate cancer growth and invasion by HDAC inhibitor SAHA. HDAC1-5 showed increased expression using a combination of DNA microarray, in-situ hybridization, and immunohistochemistry in benign and malignant human prostate tissue as well as RT-PCR and Western blot analysis on various PCa cell lines. Importantly, HDAC inhibitor SAHA suppressed, in particular, prostate cancer cell growth and invasion determined using cell proliferation and Matrigel invasion assays. The findings of this study show that the expression of HDACs and their associated corepressors are increased in prostate cancer in humans and HDAC inhibitor SAHA could serve as a potential therapeutic agent in prostate cancer in addition to anti-androgens. |
| Keywords: | immunohistochemistry; controlled study; human cell; androgen; cell proliferation; gene overexpression; reverse transcription polymerase chain reaction; antineoplastic activity; cancer cell culture; enzyme activity; prostate cancer; cancer invasion; in situ hybridization; cancer inhibition; western blotting; vorinostat; dna microarray; matrigel; microarray; hormone receptor; histone deacetylase; corepressor; histone deacetylase 1; histone deacetylase 2; histone deacetylase 3; histone deacetylase 4; histone deacetylase 5 |
| Journal Title: | American Journal of Translational Research |
| Volume: | 1 |
| Issue: | 1 |
| ISSN: | 1943-8141 |
| Publisher: | e-Century Publishing Corporation |
| Date Published: | 2009-01-01 |
| Start Page: | 62 |
| End Page: | 71 |
| Language: | English |
| PROVIDER: | scopus |
| PMCID: | PMC2776287 |
| PUBMED: | 19966939 |
| DOI/URL: | |
| Notes: | --- - "Export Date: 30 November 2010" - "Source: Scopus" |
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