First-in-humans imaging with (89)Zr-Df-IAB22M2C anti-CD8 minibody in patients with solid malignancies: Preliminary pharmacokinetics, biodistribution, and lesion targeting Journal Article

Authors: Pandit-Taskar, N.; Postow, M. A.; Hellmann, M. D.; Harding, J. J.; Barker, C. A.; O'Donoghue, J. A.; Ziolkowska, M.; Ruan, S.; Lyashchenko, S. K.; Tsai, F.; Farwell, M.; Mitchell, T. C.; Korn, R.; Le, W.; Lewis, J. S.; Weber, W. A.; Behera, D.; Wilson, I.; Gordon, M.; Wu, A. M.; Wolchok, J. D.
Article Title: First-in-humans imaging with (89)Zr-Df-IAB22M2C anti-CD8 minibody in patients with solid malignancies: Preliminary pharmacokinetics, biodistribution, and lesion targeting
Abstract: Immunotherapy is becoming the mainstay for treatment of a variety of malignancies, but only a subset of patients responds to treatment. Tumor-infiltrating CD8-positive (CD8+) T lymphocytes play a central role in antitumor immune responses. Noninvasive imaging of CD8+ T cells may provide new insights into the mechanisms of immunotherapy and potentially predict treatment response. We are studying the safety and utility of 89Zr-IAB22M2C, a radiolabeled minibody against CD8+ T cells, for targeted imaging of CD8+ T cells in patients with cancer. Methods: The initial dose escalation phase of this first-in-humans prospective study included 6 patients (melanoma, 1; lung, 4; hepatocellular carcinoma, 1). Patients received approximately 111 MBq (3 mCi) of 89Zr-IAB22M2C (at minibody mass doses of 0.2, 0.5, 1.0, 1.5, 5, or 10 mg) as a single dose, followed by PET/CT scans at approximately 1-2, 6-8, 24, 48, and 96-144 h after injection. Biodistribution in normal organs, lymph nodes, and lesions was evaluated. In addition, serum samples were obtained at approximately 5, 30, and 60 min and later at the times of imaging. Patients were monitored for safety during infusion and up to the last imaging time point. Results:89Zr-IAB22M2C infusion was well tolerated, with no immediate or delayed side effects observed after injection. Serum clearance was typically biexponential and dependent on the mass of minibody administered. Areas under the serum time-activity curve, normalized to administered activity, ranged from 1.3 h/L for 0.2 mg to 8.9 h/L for 10 mg. Biodistribution was dependent on the minibody mass administered. The highest uptake was always in spleen, followed by bone marrow. Liver uptake was more pronounced with higher minibody masses. Kidney uptake was typically low. Prominent uptake was seen in multiple normal lymph nodes as early as 2 h after injection, peaking by 24-48 h after injection. Uptake in tumor lesions was seen on imaging as early as 2 h after injection, with most 89Zr-IAB22M2C-positive lesions detectable by 24 h. Lesions were visualized early in patients receiving treatment, with SUV ranging from 5.85 to 22.8 in 6 target lesions. Conclusion:89Zr-IAB22M2C imaging is safe and has favorable kinetics for early imaging. Biodistribution suggests successful targeting of CD8+ T-cell-rich tissues. The observed targeting of tumor lesions suggests this may be informative for CD8+ T-cell accumulation within tumors. Further evaluation is under way. © 2020 by the Society of Nuclear Medicine and Molecular Imaging.
Keywords: melanoma; lung cancer; cd8+ t cell; minibody; 89zr-iab22m2c pet
Journal Title: Journal of Nuclear Medicine
Volume: 61
Issue: 4
ISSN: 0161-5505
Publisher: Society of Nuclear Medicine  
Date Published: 2020-04-01
Start Page: 512
End Page: 519
Language: English
DOI: 10.2967/jnumed.119.229781
PUBMED: 31586002
PROVIDER: scopus
PMCID: PMC7198374
Notes: Article -- Export Date: 1 May 2020 -- Source: Scopus
Citation Impact
MSK Authors
  1. Jedd D Wolchok
    805 Wolchok
  2. Michael Andrew Postow
    263 Postow
  3. James Joseph Harding
    140 Harding
  4. Christopher Barker
    161 Barker
  5. Shutian Ruan
    50 Ruan
  6. Jason S Lewis
    345 Lewis
  7. Matthew David Hellmann
    326 Hellmann
  8. Wolfgang Andreas Weber
    170 Weber