| Abstract: |
Purified murine lymphocyte pore-forming protein (PFP or perforin) was partially sequenced. Oligonucleotides synthesized on the basis of this sequence information were used to screen a murine cytotoxic T lymphocyte (CTL) cDNA library. Seven clones were obtained, two of which were sequenced, providing full-length sequence information on PFP. Murine PFP (534 a.a.) is 68% identical to human PFP. Hydropathic analysis revealed a predominantly hydrophilic protein with some hydrophobic domains, including a region (a.a. 191-251) that could contain putative membrane-spanning domains. PFP is approx. 20% identical to human C7, C8 and C9 within a region encompassing 270 a.a., confirming previous immunological cross-reactivity studies. Northern blot analysis showed that expression of PFP but not of a serine esterase transcript is enhanced in a CTL line by antigen receptor-stimulation. Southern blot analysis of mouse genomic DNA indicated that PFP is encoded as a single-copy gene with the coding region contained within 10 kilobases of genomic DNA. © 1989 Academic Press, Inc. |
| Keywords: |
nonhuman; protein conformation; t lymphocyte; animal cell; mouse; animal; mice; membrane proteins; molecular cloning; cloning, molecular; dna; amino acid sequence; molecular sequence data; t-lymphocytes, cytotoxic; base sequence; perforin; dna sequence; nucleic acid hybridization; protein structure; blotting, northern; blotting, southern; priority journal; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; genes, structural
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