Immunofluorescent monoclonal antibody detection of breast cancer in bone marrow: Sensitivity in a model system Journal Article
| Authors: | Osborne, M. P.; Asina, S.; Wong, G. Y.; Old, L. J.; Cote, R. J. |
| Article Title: | Immunofluorescent monoclonal antibody detection of breast cancer in bone marrow: Sensitivity in a model system |
| Abstract: | We have previously shown that occult micrometastases can be detected in the bone marrow of breast cancer patients using a panel of epithelial specific monoclonal antibodies in an indirect immunofluorescent assay. The sensitivity of this assay has been examined using cells from an established human breast cancer cell line (MCF-7) mixed with normal bone marrow at various dilutions from 400 cancer cells/106 marrow cells (400:116) to 10-106. MCF-7 cells were detected at the lowest concentration studied, namely 10:106. The number of fluorescent labeled MCF-7 cells counted at each concentration was related to the concentration by a simple nonlinear statistical model. At the concentration of 10:106, the model shows that this technique has the sensitivity to detect between two and four MCF-7 cells 95% of the time. Moreover, by extrapolation, the model predicts that even at the very low concentration of 2:106, there is a 95% chance of detecting one cancer cell. Therefore, this assay may be a very sensitive method for detecting cancer cells in the bone marrow in vivo. © 1989, American Association for Cancer Research. All rights reserved. |
| Keywords: | human cell; methodology; breast cancer; biological model; statistics; bone marrow; tumor cells, cultured; breast neoplasms; fluorescent antibody technique; antibodies, monoclonal; tumor cell line; neoplasm metastasis; cytochemistry; bone marrow metastasis; human; female; priority journal; support, non-u.s. gov't |
| Journal Title: | Cancer Research |
| Volume: | 49 |
| Issue: | 9 |
| ISSN: | 0008-5472 |
| Publisher: | American Association for Cancer Research |
| Date Published: | 1989-05-01 |
| Start Page: | 2510 |
| End Page: | 2513 |
| Language: | English |
| PUBMED: | 2650865 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 14 April 2020 -- Source: Scopus |
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