Selective gelatinase inhibitor peptide is effective in targeting tongue carcinoma cell tumors In Vivo Journal Article

Authors: Suojanen, J.; Vilen, S. T.; Nyberg, P.; Heikkilä, P.; Penate-Medina, O.; Saris, P. E. J.; Hagström, J.; Ranta, T. M.; Salo, T.; Sorsa, T.; Reunanen, J.
Article Title: Selective gelatinase inhibitor peptide is effective in targeting tongue carcinoma cell tumors In Vivo
Abstract: Background: Matrix metalloproteinases (MMP) are strongly associated with cancer progession. Broadspectrum MMP inhibition is rarely beneficial clinically due to adverse effects. Of all MMPs, the gelatinases are associated with the spread of several types of cancer, including oral carcinoma. We have developed gelatinase-specific peptides, as well as their fusion with green fluorescent protein (GFP), capable of effectively targeting carcinomas. Materials and Methods: Effects on tumor growth and lymphatic micrometastatic spread in vivo was studied by use of HSC-3-cell xenografted athymic nude mice. Antigelatinolytic mono- vs. polytherapies, as well as biological activity of peptide-GFP fusion, were also analyzed in vivo. Results: Antigelatinolytic therapy effectively inhibited growth of xenografted tumors in mice but the proportion of enlarged lymph nodes remained the same; antigelatinolytic polytherapy seemed not to potentiate the antitumor effects. The peptide-GFP chimera sustained its activity in vivo and effectively homed to the primary tumors. Conclusion: Peptide gelatinase inhibitors are effective in inhibiting primary tumor growth but alone do not prevent the spread of carcinoma cells; however, their bioactive GFP fusion is a candidate for tumor characterization and imaging.
Keywords: controlled study; unclassified drug; human cell; nonhuman; antineoplastic agent; cell proliferation; mouse; animals; mice; green fluorescent protein; animal experiment; animal model; in vivo study; antineoplastic activity; cancer cell culture; cytotoxicity; tumor xenograft; tumor cells, cultured; peptide; xenograft; mice, nude; enzyme inhibitors; peptide fragments; micrometastasis; green fluorescent proteins; tongue neoplasms; tongue carcinoma; matrix metalloproteinases; gelatinases; matrix metalloproteinase 9; gelatinase; diethylene triamine pentaacetic acid cyclo in 111; diethylene triamine pentaacetic acid cyclo in 111 green fluorescent protein; gelatinase inhibitor
Journal Title: Anticancer Research
Volume: 31
Issue: 11
ISSN: 0250-7005
Publisher: International Institute of Anticancer Research  
Date Published: 2011-11-01
Start Page: 3659
End Page: 3664
Language: English
PROVIDER: scopus
PUBMED: 22110184
Notes: --- - "Export Date: 3 January 2012" - "CODEN: ANTRD" - "Source: Scopus"