The role of marrow accessory cell populations in the augmentation of human erythroid progenitor cell (BFU-E) proliferation by prostaglandin E Journal Article

  

Authors:	Nocka, K. H.; Ottman, O. G.; Pelus, L. M.
Article Title:	The role of marrow accessory cell populations in the augmentation of human erythroid progenitor cell (BFU-E) proliferation by prostaglandin E
Abstract:	The requirement for CD8+ T lymphocytes in the stimulation of erythroid progenitor cells by prostaglandin E (PGE) was examined. When low density bone marrow (LD-BM) or non-adherent bone marrow (NA-BM) cells were depleted of CD8+ cells the enhancing effect of PGE on BFU-E proliferation was abrogated. However, further enrichment of marrow progenitor cells by depletion of accessory cells using a cocktail of specific monoclonal antibodies, immunoadherence and fluorescence activated cell sorting with the MY10 monoclonal antibody resulted in a population of erythroid progenitor cells which were responsive to the enhancing effect of PGE despite the absence of CD8+ cells. Stepwise individual cell lineage depletion of marrow cell populations indicated that prostaglandin E enhanced erythroid burst formation in the absence of CD8+ cells provided that glycophorin-A+ cells were removed from LD-BM or NA-BM cells. These results suggest that nucleated erythroid cell populations may modulate the enhancement of BFU-E by PGE. The ability of GP-A+ cells to block the enhancement of erythroid burst formation by PGE following removal of CD8+ T cells was confirmed by readdition of conditioned medium prepared from positively selected GP-A+ marrow cells. These results expand the role of CD8+ T cells in the PGE enhancement of BFU-E proliferation and suggest another mechanism by which accessory cells regulate the proliferation of BFU-E in bone marrow. © 1989.
Keywords:	human cell; cd8 antigen; t lymphocyte; t-lymphocytes; bone marrow; erythroblast; erythropoiesis; culture medium; drug effect; physiology; cell culture; indometacin; prostaglandins; hematopoietic stem cells; cell count; erythroblasts; hematopoietic stem cell; prostaglandin e; prostaglandins e; cell adhesion; antigen presenting cell; antigen-presenting cells; prostaglandin e1; lymphocyte depletion; normal human; culture media; growth substances; burst forming unit e; growth promotor; human; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; accessory cell; bone marrow culture; bfu-e erythroid cells; cd8+ cells
Journal Title:	Leukemia Research
Volume:	13
Issue:	7
ISSN:	0145-2126
Publisher:	Elsevier Ltd
Date Published:	1989-01-01
Start Page:	527
End Page:	534
Language:	English
DOI:	10.1016/0145-2126(89)90119-7
PUBMED:	2788237
PROVIDER:	scopus
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-0024473058&doi=10.1016%2f0145-2126%2889%2990119-7&partnerID=40&md5=4686dbc01d99da71e6072217c28a31dd
Notes:	Article -- Export Date: 14 April 2020 -- Source: Scopus

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