Modulation of the c-myb, c-myc and p53 mRNA and protein levels during induced murine erythroleukemia cell differentiation Journal Article
| Authors: | Richon, V. M.; Ramsay, R. G.; Rifkind, R. A.; Marks, P. A. |
| Article Title: | Modulation of the c-myb, c-myc and p53 mRNA and protein levels during induced murine erythroleukemia cell differentiation |
| Abstract: | The induction of murine erythroleukemia cells (MELC) to terminal differentiation by hexamethylene bisacetamide (HMBA) is accompanied by changes in the levels of c-myb and c-myc mRNA, and in p53 protein levels. We simultaneously examined the effects of HMBA on modulation of c-myb, c-myc and p53 mRNA and protein levels, and examined the relationship between these changes and commitment to terminal cell division. In MELC cultured with HMBA, c-myb protein levels paralleled c-myb mRNA levels except at 24 h, when the protein level was equivalent to the level in control cultures, whereas the mRNA had decreased. The c-myc protein paralleled c-myc mRNA throughout induction. The p53 mRNA and protein behaved in a discordant fashion. The p53 protein decreased to very low levels between 4 and 8 h and remained low, while the mRNA, which initially decreased, reaccumulated by 24 and 48 h. Transfer of MELC after 12 to 48 h of culture with HMBA to medium without inducer resulted in rapid (< 3 h) reaccumulation of the c-myb mRNA, c-myb protein, and p53 protein, and cessation of recruitment of cells to commitment. Cells already induced to commit to terminal differentiation continued to express the differentiated phenotype. |
| Keywords: | proto-oncogene proteins; nonhuman; animal cell; mouse; animal; mice; neoplasm proteins; protein; dexamethasone; cell differentiation; protein p53; cell culture; messenger rna; rna, messenger; phosphoproteins; oncogene c myc; proto-oncogene proteins c-myc; oncogene c myb; proto-oncogene proteins c-myb; leukemia, erythroblastic, acute; erythroleukemia; hexamethylenebisacetamide; acetamides; priority journal; support, non-u.s. gov't; support, u.s. gov't, p.h.s. |
| Journal Title: | Oncogene |
| Volume: | 4 |
| Issue: | 2 |
| ISSN: | 0950-9232 |
| Publisher: | Nature Publishing Group |
| Date Published: | 1989-02-01 |
| Start Page: | 165 |
| End Page: | 173 |
| Language: | English |
| PUBMED: | 2648254 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 14 April 2020 -- Source: Scopus |
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