Multiple functions of human single-stranded-DNA binding protein in simian virus 40 DNA replication: Single-strand stabilization and stimulation of DNA polymerases α and δ Journal Article
| Authors: | Kenny, M. K.; Lee, S. H.; Hurwitz, J. |
| Article Title: | Multiple functions of human single-stranded-DNA binding protein in simian virus 40 DNA replication: Single-strand stabilization and stimulation of DNA polymerases α and δ |
| Abstract: | The human single-stranded-DNA binding protein (human SSB) is required for simian virus 40 (SV40) DNA replication in vitro. SV40 larger tumor antigen and human SSB can support extensive unwinding of SV40 origin-containing DNA in the presence of ATP and a topoisomerase that relieves positive superhelicity. Although SSBs from viral and prokaryotic sources substituted for human SSB in the DNA-unwinding reaction, they did not substitute in the replication of SV40 DNA. The specificity for human SSB in SV40 DNA replication can be explained, at least in part, by the finding that DNA polymerase α was stimulated 10-fold by human SSB but not by other SSBs. Human SSB also stimulated proliferating-cell nuclear antigen-dependent DNA polymerase δ; however, other SSBs stimulated this polymerase as well. |
| Keywords: | dna binding protein; dna-binding proteins; dna replication; enzyme activation; hela cells; kinetics; simian virus 40; dna, viral; single stranded dna; dna, single-stranded; dna unwinding; dna denaturation; dna-directed dna polymerase; dna directed dna polymerase alpha; dna directed dna polymerase delta; prokaryota; single stranded dna binding protein; simiae; dna polymerase iii; dna polymerase ii; simian virus; human; priority journal; article; support, u.s. gov't, p.h.s.; proliferating-cell nuclear antigen; simian virus 40 large tumor antigen |
| Journal Title: | Proceedings of the National Academy of Sciences of the United States of America |
| Volume: | 86 |
| Issue: | 24 |
| ISSN: | 0027-8424 |
| Publisher: | National Academy of Sciences |
| Date Published: | 1989-12-01 |
| Start Page: | 9757 |
| End Page: | 9761 |
| Language: | English |
| DOI: | 10.1073/pnas.86.24.9757 |
| PUBMED: | 2557626 |
| PROVIDER: | scopus |
| PMCID: | PMC298580 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 14 April 2020 -- Source: Scopus |
