BRAF mutation analysis of fine-needle aspiration biopsies of papillary thyroid carcinoma: Impact on diagnosis and prognosis Journal Article


Authors: Colanta, A.; Lin, O.; Tafe, L.; Ghossein, R.; Nafa, K.; Mitchell, T.; Ladanyi, M.; Arcila, M.
Article Title: BRAF mutation analysis of fine-needle aspiration biopsies of papillary thyroid carcinoma: Impact on diagnosis and prognosis
Abstract: Objective: The BRAF V600E mutation has been associated with aggressive disease in papillary thyroid carcinoma (PTC). Molecular testing has been proposed as a useful adjunct to cytology in the diagnosis of malignancy and for tailoring clinical management. The aims of our study were to evaluate the BRAF mutational status using archived fine-needle aspiration biopsy (FNAB) material from patients with long-term follow-up and to correlate it with the original cytology diagnosis, clinicopathological stage at surgery, and prognosis. Study Design: FNAB material from 52 cases of PTC, with a mean follow-up of 8.4 years, was used in this study. DNA was extracted from archival cytology slides. Mutation analysis was performed by standard sequencing and locked nucleic acid-PCR/sequencing. Results: The BRAF V600E mutation was present in 46% of cases, but it was absent in all FNABs diagnosed originally as atypical and in 14 of 17 suspicious cases. Recurrence was significantly more frequent (p = 0.006) in cases with BRAF mutations and 54% of these cases presented with stage 2 or higher. Conclusion: The BRAF V600E mutation is associated with a higher pathological stage at surgery and a higher rate of recurrence. BRAF mutation analysis did not provide a significant increase in the accuracy of thyroid FNABs diagnosed as suspicious or atypical in our institution. © 2011 S. Karger AG, Basel.
Keywords: adult; human tissue; gene mutation; major clinical study; cancer recurrence; follow up; genetic analysis; polymerase chain reaction; cytology; dna; dna sequence; thyroid carcinoma; papillary carcinoma; aspiration biopsy; b raf kinase; thyroid; braf; papillary thyroid carcinoma
Journal Title: Acta Cytologica
Volume: 55
Issue: 6
ISSN: 0001-5547
Publisher: S. Karger AG  
Date Published: 2011-12-01
Start Page: 563
End Page: 569
Language: English
DOI: 10.1159/000333272
PROVIDER: scopus
PUBMED: 22156467
DOI/URL:
Notes: --- - "Cited By (since 1996): 1" - "Export Date: 3 January 2012" - "CODEN: ACYTA" - "Source: Scopus"
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MSK Authors
  1. Ronald A Ghossein
    302 Ghossein
  2. Khedoudja Nafa
    184 Nafa
  3. Oscar Lin
    231 Lin
  4. Laura Justine Tafe
    18 Tafe
  5. Marc Ladanyi
    879 Ladanyi
  6. Maria Eugenia Arcila
    350 Arcila