BRAF mutation analysis of fine-needle aspiration biopsies of papillary thyroid carcinoma: Impact on diagnosis and prognosis Journal Article
| Authors: | Colanta, A.; Lin, O.; Tafe, L.; Ghossein, R.; Nafa, K.; Mitchell, T.; Ladanyi, M.; Arcila, M. |
| Article Title: | BRAF mutation analysis of fine-needle aspiration biopsies of papillary thyroid carcinoma: Impact on diagnosis and prognosis |
| Abstract: | Objective: The BRAF V600E mutation has been associated with aggressive disease in papillary thyroid carcinoma (PTC). Molecular testing has been proposed as a useful adjunct to cytology in the diagnosis of malignancy and for tailoring clinical management. The aims of our study were to evaluate the BRAF mutational status using archived fine-needle aspiration biopsy (FNAB) material from patients with long-term follow-up and to correlate it with the original cytology diagnosis, clinicopathological stage at surgery, and prognosis. Study Design: FNAB material from 52 cases of PTC, with a mean follow-up of 8.4 years, was used in this study. DNA was extracted from archival cytology slides. Mutation analysis was performed by standard sequencing and locked nucleic acid-PCR/sequencing. Results: The BRAF V600E mutation was present in 46% of cases, but it was absent in all FNABs diagnosed originally as atypical and in 14 of 17 suspicious cases. Recurrence was significantly more frequent (p = 0.006) in cases with BRAF mutations and 54% of these cases presented with stage 2 or higher. Conclusion: The BRAF V600E mutation is associated with a higher pathological stage at surgery and a higher rate of recurrence. BRAF mutation analysis did not provide a significant increase in the accuracy of thyroid FNABs diagnosed as suspicious or atypical in our institution. © 2011 S. Karger AG, Basel. |
| Keywords: | adult; human tissue; gene mutation; major clinical study; cancer recurrence; follow up; genetic analysis; polymerase chain reaction; cytology; dna; dna sequence; thyroid carcinoma; papillary carcinoma; aspiration biopsy; b raf kinase; thyroid; braf; papillary thyroid carcinoma |
| Journal Title: | Acta Cytologica |
| Volume: | 55 |
| Issue: | 6 |
| ISSN: | 0001-5547 |
| Publisher: | S. Karger AG |
| Date Published: | 2011-12-01 |
| Start Page: | 563 |
| End Page: | 569 |
| Language: | English |
| DOI: | 10.1159/000333272 |
| PROVIDER: | scopus |
| PUBMED: | 22156467 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 1" - "Export Date: 3 January 2012" - "CODEN: ACYTA" - "Source: Scopus" |
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