Oxidative stress regulates progenitor behavior and cortical neurogenesis Journal Article
| Authors: | Chui, A.; Zhang, Q.; Dai, Q.; Shi, S. H. |
| Article Title: | Oxidative stress regulates progenitor behavior and cortical neurogenesis |
| Abstract: | Orderly division of radial glial progenitors (RGPs) in the developing mammalian cerebral cortex generates deep and superficial layer neurons progressively. However, the mechanisms that control RGP behavior and precise neuronal output remain elusive. Here, we show that the oxidative stress level progressively increases in the developing mouse cortex and regulates RGP behavior and neurogenesis. As development proceeds, numerous gene pathways linked to reactive oxygen species (ROS) and oxidative stress exhibit drastic changes in RGPs. Selective removal of PRDM16, a transcriptional regulator highly expressed in RGPs, elevates ROS level and induces expression of oxidative stressresponsive genes. Coinciding with an enhanced level of oxidative stress, RGP behavior was altered, leading to abnormal deep and superficial layer neuron generation. Simultaneous expression of mitochondrially targeted catalase to reduce cellular ROS levels significantly suppresses cortical defects caused by PRDM16 removal. Together, these findings suggest that oxidative stress actively regulates RGP behavior to ensure proper neurogenesis in the mammalian cortex. © 2020 Published by The Company of Biologists Ltd. |
| Keywords: | oxidative stress; cortical development; neocortical progenitor cells; neurogenesis transition |
| Journal Title: | Development |
| Volume: | 147 |
| Issue: | 5 |
| ISSN: | 0950-1991 |
| Publisher: | Company of Biologists |
| Date Published: | 2020-03-01 |
| Start Page: | dev184150 |
| Language: | English |
| DOI: | 10.1242/dev.184150 |
| PUBMED: | 32041791 |
| PROVIDER: | scopus |
| PMCID: | PMC7075051 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 1 April 2020 -- Source: Scopus |
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