Use of stem cell markers in dissociated mammary populations Journal Article

  


Authors: Shelton, D. N.; Fernandez-Gonzalez, R.; Illa-Bochaca, I.; Ortiz-de-Solorzano, C.; Barcellos-Hoff, M. H.; Welm, B. E.
Article Title: Use of stem cell markers in dissociated mammary populations
Abstract: The regenerative potential of mammary epithelium facilitates assessment of the "stemness" of any epithelial subpopulation in transplantation assays. Thus, mammary tissue can be dissociated into single cells, stained for cell surface markers of interest and classified using fluorescence-activated cell sorting. The selected cells can then be transplanted into epithelium-devoided fat pads from recipient hosts. Recent publications have described markers that enrich for mammary repopulating potential. Here, we describe the materials and methods necessary to sort cells according to these markers. This approach can be used interchangeably with other cell surface markers with slight variation to the protocol.
Keywords: flow cytometry; methodology; biological marker; biological markers; mouse; animal; cytology; metabolism; animals; mice; stem cell; staining; stem cells; udder; mammary glands, animal; staining and labeling; article
Journal Title: Methods in Molecular Biology
Volume: 621
ISSN: 1064-3745
Publisher: Humana Press Inc  
Date Published: 2010-01-01
Start Page: 49
End Page: 55
Language: English
DOI: 10.1007/978-1-60761-063-2_3
PUBMED: 20405358
PROVIDER: scopus
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-77954701134&doi=10.1007%2f978-1-60761-063-2_3&partnerID=40&md5=0059d675fd5b4029de7bf9b0fe4d6532
Notes: Book Chapter 3 in "Protocols for Adult Stem Cells" (ISBN: 978-1-60761-062-5) -- Export Date: 6 March 2020 -- Source: Scopus
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MSK Authors
  1. Rodrigo Fernandez-Gonzalez
    12 Fernandez-Gonzalez
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