A semiquantitative scoring system may allow biopsy diagnosis of pulmonary large cell neuroendocrine carcinoma: Experience with tissue microarrays Journal Article
| Authors: | Baine, M. K.; Sinard, J. H.; Cai, G.; Homer, R. J. |
| Article Title: | A semiquantitative scoring system may allow biopsy diagnosis of pulmonary large cell neuroendocrine carcinoma: Experience with tissue microarrays |
| Abstract: | Objectives: The aim of this study was to devise reproducible biopsy criteria for distinguishing pulmonary large cell neuroendocrine carcinoma (LCNEC) from non-small cell lung carcinoma (NSCLC). Methods: Tissue microarrays of LCNEC and NSCLC were generated from resection specimens and used as biopsy surrogates. They were stained for neuroendocrine markers, Ki-67, napsin-A, and p40, and independently analyzed by standardized morphologic criteria by four pathologists. Tumors were scored based on morphology, neuroendocrine marker expression, and Ki-67 proliferative index. Results: The average total score for LCNEC was significantly higher than for NSCLC (5.65 vs 0.51, P <. 0001). Utilizing a cutoff score of 4 or higher showed 100% sensitivity and 99% specificity for LCNEC diagnosis, with an excellent agreement among four pathologists (98%). Conclusions: The proposed semiquantitative approach based on a combination of specific morphologic and immunophenotypic features may be a useful tool for biopsy diagnosis of LCNEC. © 2019 American Society for Clinical Pathology. All rights reserved. |
| Keywords: | immunohistochemistry; adult; clinical article; controlled study; human tissue; protein expression; aged; middle aged; unclassified drug; human cell; histopathology; disease marker; sensitivity and specificity; ki 67 antigen; cell proliferation; reproducibility; cohort analysis; lung cancer; standardization; probability; scoring system; immunophenotyping; cancer tissue; tissue microarray; lung biopsy; fisher exact test; pathologist; large cell neuroendocrine carcinoma; nucleolus; non small cell lung cancer; protein p40; tissue necrosis; diagnostic test accuracy study; biopsy diagnosis; very elderly; human; male; female; priority journal; article; napsin a; large cell neuroendocrine carcinoma of the lung; scoring criteria |
| Journal Title: | American Journal of Clinical Pathology |
| Volume: | 153 |
| Issue: | 2 |
| ISSN: | 0002-9173 |
| Publisher: | Oxford University Press |
| Date Published: | 2020-02-01 |
| Start Page: | 165 |
| End Page: | 174 |
| Language: | English |
| DOI: | 10.1093/ajcp/aqz149 |
| PUBMED: | 31593583 |
| PROVIDER: | scopus |
| PMCID: | PMC7571487 |
| DOI/URL: | |
| Notes: | Article -- Source: Scopus |
