Endogenous retroviral long terminal repeats within the HLA-DQ locus Journal Article

   


Authors: Kambhu, S.; Falldorf, P.; Lee, J. S.
Article Title: Endogenous retroviral long terminal repeats within the HLA-DQ locus
Abstract: Two endogenous retroviral long terminal repeats (LTRs) were found in the human major histocompatibility complex locus HLA-DQ. The solo LTRs, unlinked to retrovirus structural genes, are located ~ 5 kilobases apart from each other and in the same transcriptional orientation, which is opposite to that for the HLA-DQB1 gene. These elements exhibit > 90% homology to the LTRs of the human endogenous retrovirus HERV-K10. The conservation of putative regulatory elements found within the LTRs and their position relative to the HLA-DQB1 gene suggest that these elements may confer distinct regulatory properties on genes in the HLA-DQ region. Polymorphic variation between different HLA haplotypes for the presence of the LTRs at this location and of the molecular architecture within this subregion is supported by polymerase chain reaction and Southern blot analysis. Comparisons of chromosomes with and without the LTRs in this region will provide a unique opportunity in the human genome to analyze transposition or integration of retroviral sequences.
Keywords: comparative study; polymerase chain reaction; cell line; b-lymphocytes; cloning, molecular; molecular sequence data; homozygote; base sequence; sequence homology, nucleic acid; major histocompatibility complex; genes, mhc class ii; repetitive sequences, nucleic acid; hla antigen; retrovirus; retroviridae; dna polymorphism; long terminal repeat; polymorphism; restriction mapping; variation (genetics); human endogenous retrovirus; hla dq antigen; oligonucleotide probes; unidentified retrovirus; human; priority journal; article; polymorphism (genetics); support, non-u.s. gov't; support, u.s. gov't, p.h.s.; hla-dq antigens; cosmids
Journal Title: Proceedings of the National Academy of Sciences of the United States of America
Volume: 87
Issue: 13
ISSN: 0027-8424
Publisher: National Academy of Sciences  
Date Published: 1990-07-01
Start Page: 4927
End Page: 4931
Language: English
DOI: 10.1073/pnas.87.13.4927
PUBMED: 2114643
PROVIDER: scopus
PMCID: PMC54234
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0025357760&doi=10.1073%2fpnas.87.13.4927&partnerID=40&md5=52ea28646117b2fb88fb94e6937f70b9
Notes: Article -- Export Date: 27 January 2020 -- Source: Scopus
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