Functional expression of murine multidrug resistance in Xenopus laevis oocytes Journal Article

   


Authors: Castillo, G.; Vera, J. C.; Yang, C. P. H.; Horwitz, S. B.; Rosen, O. M.
Article Title: Functional expression of murine multidrug resistance in Xenopus laevis oocytes
Abstract: The development of multidrug resistance (MDR) is associated with the overproduction of a plasma membrane glycoprotein, P glycoprotein. Here we report the functional expression of a member of the murine mdr family of proteins and show that Xenopus oocytes injected with RNA encoding the mouse mdr1b P glycoprotein develop a MDR-like phenotype. Immunological analysis indicated that oocytes injected with the mdr 1b RNA synthesized a protein with the size and immunological characteristics of the mouse mdr 1b P glycoprotein. These oocytes exhibited a decreased accumulation of [3H]vinblastine and showed an increased capacity to extrude the drug compared to control oocytes not expressing the P glycoprotein. In addition, competition experiments indicated that verapamil, vincristine, daunomycin, and quinidine, but not colchicine, can overcome the rapid drug efflux conferred by the expression of the mouse P glycoprotein. (.
Keywords: nonhuman; animal cell; oocyte; animal; mice; oocytes; gene expression; vincristine; transcription, genetic; drug resistance; vinblastine; animalia; rna; anura; amino acid sequence; molecular sequence data; kinetics; genetic engineering; cell culture; membrane glycoproteins; carrier proteins; daunorubicin; murinae; plasmids; antibodies; radioisotope; verapamil; p-glycoprotein; colchicine; xenopus laevis; glycoprotein p; mrna expression; quinidine; toad; female; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; p glycoprotein; multidrug transporter
Journal Title: Proceedings of the National Academy of Sciences of the United States of America
Volume: 87
Issue: 12
ISSN: 0027-8424
Publisher: National Academy of Sciences  
Date Published: 1990-06-01
Start Page: 4737
End Page: 4741
Language: English
DOI: 10.1073/pnas.87.12.4737
PUBMED: 1693776
PROVIDER: scopus
PMCID: PMC54192
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0025339169&doi=10.1073%2fpnas.87.12.4737&partnerID=40&md5=2cd2fea0961cc229d5dc2165389967ea
Notes: Article -- Export Date: 27 January 2020 -- Source: Scopus
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MSK Authors
  1. Juan C Vera
    64 Vera
  2. Ora Mendelsohn Rosen
    58 Rosen
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