Regulation of alternative splicing in the generation of isoforms of the mouse Ly-5 (CD45) glycoprotein Journal Article
| Authors: | Saga, Y.; Lee, J. S.; Saraiya, C.; Boyse, E. A. |
| Article Title: | Regulation of alternative splicing in the generation of isoforms of the mouse Ly-5 (CD45) glycoprotein |
| Abstract: | Alternative splicing generates various Ly-5 glycoprotein isoforms of the cell surface that typify different cell lineages and stages of hematopoietic differentiation in the mouse, exons 4-6 are incorporated to generate a B-cell isoform (b220) and excluded from a T-cell isoform (T200), the other coding exons (3 and 7-33) being shared. As a first step to understanding the mechanisms regulating Ly-5 alternative splicing, and thus determining Ly-5 isoforms, a minigene representing exons 3-7 was transfected into Ly-5-expressor T cells and B cells and into nonexpressor L cells for comparison of splicing patterns. We conclude that all the information required for faithful splice-site selection according to cell type is contained within the resulting pre-mRNA. The splicing pattern manifested by nonexpressor L cells may represent a default and nonregulated type. We postulate trans-acting factor(s) to account for the selection of appropriate exons, and we provide support for this interpretation from analysis of fused hybrid T-B cells, which exhibited B-cell specific Ly-5 transcripts. Splicing patterns were well conserved despite substantial disruption of constructs. However, extensive deletion analyses suggested that cis sequences flanking and within exon 6 affect the exclusion of that exon in T cells. |
| Keywords: | exons; nonhuman; polymerase chain reaction; t-lymphocytes; animal cell; mouse; animal; mice; gene expression; cell line; transfection; animalia; b-lymphocytes; rna; gene expression regulation; lymphocyte differentiation; molecular sequence data; membrane glycoproteins; alternative rna splicing; rna structure; chromosome deletion; isoprotein; cd45 antigen; rna splicing; antigens, differentiation; antigens, cd45; histocompatibility antigens; restriction mapping; oligonucleotide probes; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; trans-acting factors |
| Journal Title: | Proceedings of the National Academy of Sciences of the United States of America |
| Volume: | 87 |
| Issue: | 10 |
| ISSN: | 0027-8424 |
| Publisher: | National Academy of Sciences |
| Date Published: | 1990-05-01 |
| Start Page: | 3728 |
| End Page: | 3732 |
| Language: | English |
| DOI: | 10.1073/pnas.87.10.3728 |
| PUBMED: | 1692621 |
| PROVIDER: | scopus |
| PMCID: | PMC53976 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 27 January 2020 -- Source: Scopus |
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