A model of reversible obstructive jaundice in the rat Journal Article


Authors: Posner, M. C.; Burt, M. E.; Stone, M. D.; Han, B. L.; Warren, R. S.; Vydelingum, N. A.; Brennan, M. F.
Article Title: A model of reversible obstructive jaundice in the rat
Abstract: A model of reversible, extrahepatic biliary obstruction is described. Vessel loop blockade of the biliary tree results in obstructive jaundice while removal of the exteriorized vessel loop provides internal biliary drainage without subsequent laparotomy. This technique combined with a system for chronic venous infusion and arterial blood sampling in the unrestrained rat is ideal for longterm metabolic studies of obstructive jaundice. Male Fisher 344 rats (275-350 g) underwent either the combined procedure of total biliary tract blockade and vascular access or sham operation. Mean serum bilirubin was significantly elevated (12.7 ± 8.9 mg/dl) in the experimental group and following relief of biliary obstruction significantly dropped below 1 mg/dl in all animals except one. Concomitant changes in alkaline phosphatase, glutamate oxaloacetate transaminase, and glutamate pyruvate transaminase were seen. Experimental and control rats initially lost weight following laparotomy; however, mean body weight stabilized by the 5th postoperative day and was similar in both groups on the 10th postoperative day. This combined procedure is a simple, effective and reproducible method of obstructive jaundice. © 1990.
Keywords: nonhuman; animal; animal model; body weight; bilirubin; rat; rats; bile duct obstruction; liver enzyme; disease models, animal; cholestasis; rats, inbred f344; aspartate aminotransferases; alanine transaminase; male; priority journal; article; support, u.s. gov't, p.h.s.
Journal Title: Journal of Surgical Research
Volume: 48
Issue: 3
ISSN: 0022-4804
Publisher: Academic Press Inc., Elsevier Science  
Date Published: 1990-03-01
Start Page: 204
End Page: 210
Language: English
DOI: 10.1016/0022-4804(90)90214-m
PUBMED: 2314093
PROVIDER: scopus
DOI/URL:
Notes: Article -- Export Date: 27 January 2020 -- Source: Scopus
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  1. Murray F Brennan
    1059 Brennan
  2. Michael E. Burt
    187 Burt
  3. Robert S. Warren
    17 Warren