Identification and characterization of a mutation in the dihydrofolate reductase gene from the methotrexate-resistant Chinese hamster ovary cell line pro-3 Mtx(RIII) Journal Article

  


Authors: Dicker, A. P.; Volkenandt, M.; Schweitzer, B. I.; Banerjee, D.; Bertino, J. R.
Article Title: Identification and characterization of a mutation in the dihydrofolate reductase gene from the methotrexate-resistant Chinese hamster ovary cell line pro-3 Mtx(RIII)
Abstract: A methotrexate-resistant Chinese hamster ovary cell line (Pro-3 Mtx(RIII)), resistant due to a low-level amplified, altered target enzyme, dihydrofolate reductase (DHFR), has been characterized on the molecular level. The cDNA and coding regions of all six DHFR exons were amplified in vitro using Taq polymerase and directly sequenced. Analysis of the Pro-3 Mtx(RIII) DHFR cDNA demonstrated a C → T base transition at nucleotide 67 that results in the substitution of phenylalanine for leucine at residue 22 and the loss of a BsaI site in the Pro-3 Mtx(RIII) cDNA. This mutation results in a decreased binding of methotrexate to the altered enzyme. Molecular modeling of Leu22 → Phe supports the concept of the importance of Leu22 in the active site of the enzyme and indicates that replacement with phenylalanine will decrease the binding of methotrexate.
Keywords: gene mutation; mutation; nonhuman; methotrexate; animal cell; animal; gene amplification; cell line; drug resistance; animalia; ovary; dna; amino acid sequence; molecular sequence data; kinetics; cell culture; base sequence; binding sites; molecular structure; leucine; dihydrofolate reductase; tetrahydrofolate dehydrogenase; phenylalanine; cho cell; cricetinae; cricetulus griseus; dna-directed dna polymerase; hamster; female; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; hamsters; taq polymerase
Journal Title: Journal of Biological Chemistry
Volume: 265
Issue: 14
ISSN: 0021-9258
Publisher: American Society for Biochemistry and Molecular Biology  
Date Published: 1990-05-15
Start Page: 8317
End Page: 8321
Language: English
PUBMED: 2335526
PROVIDER: scopus
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0025343839&partnerID=40&md5=85d51778a0e58eab16cb5d7e1ec57f14
Notes: Source: Scopus
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MSK Authors
  1. Debabrata Banerjee
    136 Banerjee
  2. Joseph Bertino
    363 Bertino
  3. Matthias Volkenandt
    27 Volkenandt
  4. Adam P. Dicker
    15 Dicker
  5. Barry I. Schweitzer
    22 Schweitzer
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