| Abstract: |
To explore bidirectional regulatory interactions between interferons and autocrine polypeptide factors, we examined the modulation of expression of transforming growth factor-α and 2',5'-oligoadenylate synthetase activity in A431 epidermoid carcinoma cells after treatment with interferon-γ and transforming growth factor-α. Treatment of A431 cells with interferon-γ increased steady state levels of transforming growth factor-α mRNA by 4-fold and increased the levels of transforming growth factor-α in the culture medium. There were additive growth inhibitory effects upon coaddition of exogenous transforming growth factor-α and interferon-γ to the cultures. Addition of transforming growth factor-α to A431 cell cultures in the absence of interferon could stimulate the induction of 2',5'-oligoadenylate synthetase activity by more than 2-fold. These findings demonstrate that the induction of transforming growth factor-α in interferon-γ-treated A431 cells could act to regulate interferon-induced gene(s), e.g. 2',5'-oligoadenylate synthetase, suggesting interactions between a potential autocrine growth factor and the interferon system in the growth regulation of A431 cells. |
| Keywords: |
carcinoma, squamous cell; interferon; cell growth; receptor, epidermal growth factor; tumor cells, cultured; gene expression regulation; kinetics; cell culture; messenger rna; rna, messenger; growth regulation; transforming growth factors; molecular weight; isoenzymes; transforming growth factor alpha; enzyme induction; interferon type ii; priority journal; article; support, u.s. gov't, p.h.s.; 2',5'-oligoadenylate synthetase; 2',5' oligoadenylate synthetase
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