Detection of preferential NRAS mutations in human male germ cell tumors by the polymerase chain reaction Journal Article
| Authors: | Ganguly, S.; Murty, V. V. V. S.; Samaniego, F.; Reuter, V. E.; Bosl, G. J.; Chaganti, R. S. K. |
| Article Title: | Detection of preferential NRAS mutations in human male germ cell tumors by the polymerase chain reaction |
| Abstract: | We have studied 31 male germ cell tumors (GCTs) for probable mutations in codons 12, 13, and 61 of HRAS, KRAS, and NRAS oncogenes using the polymerase chain reaction. Twenty of the thirty‐one tumors exhibited NRAS gene mutations, 14 in codon 61, and six in codon 12, whereas no mutations were detected in HRAS and KRAS genes. The NRAS mutations were equally prevalent in seminomatous and nonseminomatous GCTs. Thus 13 of 22 seminomas, six of seven embryonal carcinomas, and one of two mixed tumors exhibited mutations. Two non‐seminomatous tumors (an embryonal carcinoma and a yolk sac/teratoma) had mutations in both codons 12 and 61. The high frequency of NRAS mutations observed in the present study suggests that NRAS gene products may play an important role in growth regulatory functions of premalignant and malignant germ cells. Copyright © 1990 Wiley‐Liss, Inc., A Wiley Company |
| Keywords: | human cell; mutation; polymerase chain reaction; heredity; cell growth; neoplasm proteins; gtp-binding proteins; oncogene; genetic engineering; dna, neoplasm; testicular neoplasms; neoplasms, germ cell and embryonal; retroperitoneal neoplasms; dna mutational analysis; genes, ras; germ cell tumor; embryonal carcinoma; seminoma; gtp phosphohydrolases; human; male; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; proto-oncogene protein p21(ras) |
| Journal Title: | Genes Chromosomes and Cancer |
| Volume: | 1 |
| Issue: | 3 |
| ISSN: | 1045-2257 |
| Publisher: | Wiley Periodicals, Inc |
| Date Published: | 1990-01-01 |
| Start Page: | 228 |
| End Page: | 232 |
| Language: | English |
| DOI: | 10.1002/gcc.2870010307 |
| PUBMED: | 1964583 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Source: Scopus |
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