Radiolocalization of monoclonal antibodies in hepatic metastases from human colon cancer in congenitally athymic mice Conference Paper
| Authors: | Yoshida, K.; Rivoire, M.; Divgi, C.; Welt, S.; Cohen, A. M.; Sigurdson, E. R. |
| Title: | Radiolocalization of monoclonal antibodies in hepatic metastases from human colon cancer in congenitally athymic mice |
| Conference Title: | 2nd Conference on Radioimmunodetection and Radioimmunotherapy of Cancer |
| Abstract: | Intrasplenic injection of the HT-29 LMM metastatic human colon cancer line reproducibility results in hepatic metastasis formation in congenitally athymic mice. HT-29-15, a murine monoclonal antibody mAb) of the IgG1 class reactive with the HT-29 LMM line, and BL-3, an isotype-matched control antibody, were labeled with 125I. Labeled mAbs were injected i.v. in mice with hepatic metastases, and animals were sacrificed on days 3, 5, and 7. Specific mAb uptake by tumor was significantly greater than nonspecific mAb uptake, as evidenced by specific/nonspecific tumor/blood ratios (radiolocalization indices) of 3.47/1-25.6/1. Relative mAb uptake was greater by the hepatic tumors than by the splenic tumors from day 3 to day 7, although this was significant (P < 0.05) only on day 7 (5.12 ± 2.97 versus 1.79 ± 0.71). Tumor/uninvolved tissue ratios were also significantly greater (P < 0.05) for the hepatic metastases than for the splenic tumors on day 7 (12.23 ± 3.85 versus 6.63 ± 2.63). This murine hepatic metastasis model appears useful for evaluation of localization of mAbs to hepatic metastases from human colon carcinoma. |
| Keywords: | human cell; nonhuman; conference paper; mouse; animal; mice; animal experiment; colonic neoplasms; mice, inbred balb c; monoclonal antibody; liver metastasis; antibodies, monoclonal; iodine 125; isotope labeling; nude mouse; tissue distribution; iodine radioisotopes; mice, nude; colon cancer; transplantation, heterologous; radioisotope; neoplasm transplantation; radioimmunoassay; liver neoplasms, experimental; human; female; priority journal; support, non-u.s. gov't |
| Journal Title | Cancer Research |
| Volume: | 50 |
| Issue: | 3 Suppl. |
| Conference Dates: | 1988 Sep 8-10 |
| Conference Location: | Princeton, NJ |
| ISBN: | 0008-5472 |
| Publisher: | American Association for Cancer Research |
| Date Published: | 1990-02-01 |
| Start Page: | 862s |
| End Page: | 865s |
| Language: | English |
| PUBMED: | 2297735 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Conference Paper -- Export Date: 27 January 2020 -- Source: Scopus |
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