B-cell differentiation following autologous, conventional, or T-cell depleted bone marrow transplantation: A recapitulation of normal B-cell ontogeny Journal Article

  

Authors:	Small, T. N.; Keever, C. A.; Weiner-Fedus, S.; Heller, G.; O'Reilly, R. J.; Flomenberg, N.
Article Title:	B-cell differentiation following autologous, conventional, or T-cell depleted bone marrow transplantation: A recapitulation of normal B-cell ontogeny
Abstract:	The circulating lymphocytes of 88 consecutive patients following autologous, conventional, or T-cell depleted bone marrow transplantation were serially analyzed for B-cell surface antigen expression and function. In the majority of patients, except for those who developed chronic graft-versus-host disease, the number of circulating CD20+ B cell normalized by the fourth posttransplant month. The earliest detectable B cells normally expressed HLA-DR, CD19, surface immunoglobulin (sIg), CD21, Leu-8, and lacked expression of CD10 (CALLA). In addition, the circulating B cells expressed CD1c, CD38, CD5, and CD23 for the first year following transplant, antigens that are normally expressed on a small percentage of circulating B cells in normal adults, but highly expressed on cord blood B cells. Similar to cord blood B cells, patient B cells, patient B cells isolated during the first year following transplant, proliferated normally to Staphylococcus aureus Cowan strain I (SAC), and produced IgM, but minimal or no IgG when stimulated with pokeweed mitogen and SAC, unlike normal adult B cells that produce both. The similar phenotype and function of posttransplant and cord blood B cells, and their similar rate of decline in patients and normal children adds further evidence to support the hypothesis that B-cell differentiation posttransplant is recapitulating normal B-cell ontogeny.
Keywords:	adult; child; child, preschool; human cell; major clinical study; comparative study; t-lymphocytes; cell differentiation; b-lymphocytes; antigens, cd20; lymphocyte activation; immunoglobulin g; infant, newborn; hla-dr antigens; immunophenotyping; aging; leukocyte count; antigens, cd; staphylococcus aureus; bone marrow transplantation; graft vs host disease; b lymphocyte differentiation; immunoglobulin m; antigens, differentiation, b-lymphocyte; human; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s.
Journal Title:	Blood
Volume:	76
Issue:	8
ISSN:	0006-4971
Publisher:	American Society of Hematology
Date Published:	1990-10-15
Start Page:	1647
End Page:	1656
Language:	English
PUBMED:	1698484
PROVIDER:	scopus
DOI:	10.1182/blood.V76.8.1647.1647
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-0025172230&partnerID=40&md5=bb3adbb786fae6e1fac968f47e94a4ff
Notes:	Article -- Export Date: 27 January 2020 -- Source: Scopus

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