Bovine alpha(s1)-casein gene sequences direct high level expression of active human urokinase in mouse milk Journal Article
| Authors: | Meade, H.; Gates, L.; Lacy, E.; Lonberg, N. |
| Article Title: | Bovine alpha(s1)-casein gene sequences direct high level expression of active human urokinase in mouse milk |
| Abstract: | We have produced a line of transgenic mice carrying a hybrid bovine αs1 casein/human urokinase gene. Bovine αs1-casein gene regulatory sequences specifically direct expression of the human urokinase gene in lactating mammary tissue from these mice. Urokinase is a 54 kD protein with 9 disulfide bonds that is normally synthesized in the kidney; however, the casein/urokinase transgenic mice secrete active human urokinase into their milk at concentrations of 1-2 mg/ml. The mice show no other abnormalities. They give birth to, and nurse, normal sized healthy litters. Thus it is possible to produce high concentrations of a large, cysteine rich, non-milk protein in the milk of transgenic animals. This line of transgenic mice provides a model for the eventual production of transgenic farm animals producing high levels of recombinant proteins in their milk. © 1990 Nature Publishing Group. |
| Keywords: | human cell; nonhuman; animal cell; mouse; animal; mice; gene expression; animalia; mus musculus; genetic vectors; mice, transgenic; rna; gene expression regulation; molecular cloning; molecular sequence data; recombinant fusion proteins; genetic engineering; nucleotide sequence; recombinant protein; base sequence; models, genetic; cattle; radioisotope; milk; bovinae; restriction mapping; urinary plasminogen activator; bos taurus; caseins; milk proteins; human; priority journal; article; casein |
| Journal Title: | Bio/Technology |
| Volume: | 8 |
| Issue: | 5 |
| ISSN: | 0733-222X |
| Publisher: | Nature Publishing Group |
| Date Published: | 1990-05-01 |
| Start Page: | 443 |
| End Page: | 446 |
| Language: | English |
| DOI: | 10.1038/nbt0590-443 |
| PUBMED: | 1369989 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Source: Scopus |
