General assay for phosphoproteins in cerebrospinal fluid: A candidate marker for paraneoplastic cerebellar degeneration Journal Article

  

Authors:	Gandy, S. E.; Grebb, J. A.; Rosen, N.; Albert, K. A.; Devinsky, O.; Blumberg, H.; Anderson, N.; Cedarbaum, J. M.; Porter, R. J.; Sedvall, G.; Posner, J. B.; Greengard, P.
Article Title:	General assay for phosphoproteins in cerebrospinal fluid: A candidate marker for paraneoplastic cerebellar degeneration
Abstract:	The components of protein phosphorylation systems (protein kinases, protein phosphatases, and their phosphoprotein substrates) are highly enriched in neuronal cells compared with other cell types. We exploited this relative neuronal enrichment of protein phosphorylation system components to develop a general assay technique for putative protein kinase substrates (phosphoproteins) in human cerebrospinal fluid. Using this cerebrospinal fluid phosphoprotein assay, we have detected a putative protein kinase C substrate protein of apparent Mr 60 kd in 6 of 14 patients with paraneoplastic cerebellar degeneration but not in any of 55 patients with a variety of other neurological diseases. Phosphoproteins in cerebrospinal fluid may provide novel and unique markers for the diagnosis or staging of neuronal diseases as well as offer potential insights into the biochemical characterization of affected neuronal populations. Copyright © 1990 American Neurological Association
Keywords:	adult; clinical article; human tissue; aged; aged, 80 and over; biological markers; cerebrospinal fluid; phosphoproteins; protein kinase c; paraneoplastic syndrome; phosphoprotein; autoradiography; electrophoresis, polyacrylamide gel; middle age; cerebellar diseases; paraneoplastic syndromes; cerebellum degeneration; human; male; female; priority journal; article; support, non-u.s. gov't; support, u.s. gov't, p.h.s.; support, u.s. gov't, non-p.h.s.
Journal Title:	Annals of Neurology
Volume:	28
Issue:	6
ISSN:	0364-5134
Publisher:	Wiley Blackwell
Date Published:	1990-12-01
Start Page:	829
End Page:	833
Language:	English
DOI:	10.1002/ana.410280616
PUBMED:	2285268
PROVIDER:	scopus
DOI/URL:	https://www.scopus.com/inward/record.uri?eid=2-s2.0-0025613871&doi=10.1002%2fana.410280616&partnerID=40&md5=e87339d16a00a48ff8807b2ce37598bc
Notes:	Article -- Source: Scopus

https://synapse.mskcc.org/synapse/works/has_related_data_chk/159066